261841

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 128552..130120 Replicon plasmid unnamed1
Accession NZ_CP107721
Organism Escherichia coli strain 2021CK-01361

Toxin (Protein)


Gene name hipA Uniprot ID A0A859R327
Locus tag N4T39_RS25165 Protein ID WP_024134513.1
Coordinates 128813..130120 (+) Length 436 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A859R2S1
Locus tag N4T39_RS25160 Protein ID WP_039272366.1
Coordinates 128552..128803 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4T39_RS25130 (N4T39_25130) 124205..124930 + 726 WP_001553093.1 hypothetical protein -
N4T39_RS25135 (N4T39_25135) 124986..125438 + 453 WP_001553092.1 hypothetical protein -
N4T39_RS25140 (N4T39_25140) 125454..126056 - 603 WP_039272372.1 hypothetical protein -
N4T39_RS25145 (N4T39_25145) 126317..126598 + 282 WP_000575656.1 hypothetical protein -
N4T39_RS25150 (N4T39_25150) 126617..127180 + 564 WP_001132404.1 hypothetical protein -
N4T39_RS25155 (N4T39_25155) 127183..128187 + 1005 WP_039272368.1 tyrosine-type recombinase/integrase -
N4T39_RS25160 (N4T39_25160) 128552..128803 + 252 WP_039272366.1 helix-turn-helix domain-containing protein Antitoxin
N4T39_RS25165 (N4T39_25165) 128813..130120 + 1308 WP_024134513.1 type II toxin-antitoxin system HipA family toxin Toxin
N4T39_RS25170 (N4T39_25170) 130117..130611 + 495 WP_039272364.1 hypothetical protein -
N4T39_RS25175 (N4T39_25175) 130786..131724 - 939 WP_044596315.1 hypothetical protein -
N4T39_RS25180 (N4T39_25180) 131923..132141 + 219 Protein_155 PAAR domain-containing protein -
N4T39_RS25185 (N4T39_25185) 132334..133563 + 1230 WP_228279877.1 DUF2235 domain-containing protein -
N4T39_RS25190 (N4T39_25190) 133935..134237 - 303 WP_031611417.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / mph(A) - 1..145946 145946


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-64)


Sequences

Toxin        


Download         Length: 436 a.a.        Molecular weight: 47950.12 Da        Isoelectric Point: 6.2444

>T261841 WP_024134513.1 NZ_CP107721:128813-130120 [Escherichia coli]
MQTLTVAMNGEVVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQARFR
VETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIEQLLEGYQEAPLGMAEDADFRISIAGAQEKTALL
WYQDRWQRPLGSTPTSHIFKLPIGKIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWSKS
GEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEPAS
SFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYHFAMIQPRHFISTAAHVGFPQDTAAQLMFDMATKTEEVVA
TVAAELPAGFPEHISSAIFEGLSSQAAKILKGVAK

Download         Length: 1308 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9369.79 Da        Isoelectric Point: 9.8930

>AT261841 WP_039272366.1 NZ_CP107721:128552-128803 [Escherichia coli]
MKVTSPSLLAAAVRDQRKLSKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLSALDLELHVVKRGSTLDDSKVWD
REW

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A859R327

Antitoxin

Source ID Structure
AlphaFold DB A0A859R2S1

References