Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4508220..4508717 Replicon chromosome
Accession NZ_CP107720
Organism Escherichia coli strain 2021CK-01361

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N4T39_RS22180 Protein ID WP_001173343.1
Coordinates 4508220..4508393 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag N4T39_RS22185 Protein ID WP_001425728.1
Coordinates 4508421..4508717 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4T39_RS22170 (4504890) 4504890..4506347 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
N4T39_RS22175 (4506584) 4506584..4508101 + 1518 WP_001295074.1 lysine--tRNA ligase -
N4T39_RS22180 (4508220) 4508220..4508393 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N4T39_RS22185 (4508421) 4508421..4508717 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N4T39_RS22190 (4508944) 4508944..4509216 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
N4T39_RS22195 (4509228) 4509228..4509458 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
N4T39_RS22200 (4509639) 4509639..4511270 + 1632 WP_001216486.1 sensor histidine kinase -
N4T39_RS22205 (4511267) 4511267..4511986 + 720 WP_000611288.1 two-component system response regulator DcuR -
N4T39_RS22210 (4512313) 4512313..4513293 + 981 WP_000082736.1 IS5-like element ISEc68 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4512313..4513293 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T261840 WP_001173343.1 NZ_CP107720:c4508393-4508220 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT261840 WP_001425728.1 NZ_CP107720:c4508717-4508421 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References