Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4316188..4316764 Replicon chromosome
Accession NZ_CP107720
Organism Escherichia coli strain 2021CK-01361

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag N4T39_RS21230 Protein ID WP_001297643.1
Coordinates 4316477..4316764 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag N4T39_RS21225 Protein ID WP_000063160.1
Coordinates 4316188..4316490 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4T39_RS21210 (4312784) 4312784..4314934 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
N4T39_RS21215 (4314984) 4314984..4315187 + 204 WP_000467859.1 YbdD/YjiX family protein -
N4T39_RS21220 (4315198) 4315198..4316154 + 957 WP_001297640.1 GTPase -
N4T39_RS21225 (4316188) 4316188..4316490 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
N4T39_RS21230 (4316477) 4316477..4316764 - 288 WP_001297643.1 BrnT family toxin Toxin
N4T39_RS21235 (4316963) 4316963..4317659 - 697 Protein_4163 IS1 family transposase -
N4T39_RS21240 (4317974) 4317974..4319206 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
N4T39_RS21245 (4319247) 4319247..4320527 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4316963..4317172 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T261838 WP_001297643.1 NZ_CP107720:c4316764-4316477 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT261838 WP_000063160.1 NZ_CP107720:c4316490-4316188 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References