Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3828942..3829621 Replicon chromosome
Accession NZ_CP107720
Organism Escherichia coli strain 2021CK-01361

Toxin (Protein)


Gene name ykfI Uniprot ID S1FHS4
Locus tag N4T39_RS18695 Protein ID WP_000854680.1
Coordinates 3829280..3829621 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EWR7
Locus tag N4T39_RS18690 Protein ID WP_000070396.1
Coordinates 3828942..3829259 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4T39_RS18645 (3824380) 3824380..3825201 + 822 WP_000197388.1 DUF932 domain-containing protein -
N4T39_RS18650 (3825418) 3825418..3826119 + 702 WP_060616110.1 WYL domain-containing protein -
N4T39_RS18655 (3826160) 3826160..3826396 + 237 WP_001144031.1 protein YpjK -
N4T39_RS18660 (3826396) 3826396..3826839 + 444 WP_000649865.1 hypothetical protein -
N4T39_RS18665 (3826862) 3826862..3827329 + 468 WP_001385283.1 protein YkfB -
N4T39_RS18670 (3827406) 3827406..3827645 + 240 WP_000194654.1 DUF905 family protein -
N4T39_RS18675 (3827743) 3827743..3828201 + 459 WP_000211838.1 antirestriction protein -
N4T39_RS18680 (3828217) 3828217..3828693 + 477 WP_001549015.1 RadC family protein -
N4T39_RS18685 (3828702) 3828702..3828923 + 222 WP_000691994.1 DUF987 domain-containing protein -
N4T39_RS18690 (3828942) 3828942..3829259 + 318 WP_000070396.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
N4T39_RS18695 (3829280) 3829280..3829621 + 342 WP_000854680.1 type IV toxin-antitoxin system toxin YkfI Toxin
N4T39_RS18700 (3830540) 3830540..3831778 + 1239 WP_000219087.1 IS110-like element ISEsa2 family transposase -
N4T39_RS18705 (3831935) 3831935..3833401 - 1467 WP_000723924.1 hypothetical protein -
N4T39_RS18710 (3833717) 3833717..3834106 - 390 WP_223596848.1 hypothetical protein -
N4T39_RS18715 (3834350) 3834350..3834436 - 87 Protein_3670 capsid protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12892.95 Da        Isoelectric Point: 9.6543

>T261835 WP_000854680.1 NZ_CP107720:3829280-3829621 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11982.62 Da        Isoelectric Point: 7.0270

>AT261835 WP_000070396.1 NZ_CP107720:3828942-3829259 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHS4

Antitoxin

Source ID Structure
AlphaFold DB S1EWR7

References