Detailed information of TA system TA026174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1071772..1072372
• Replicon: chromosome
• Accession: NC_017033
• Organism: Frateuria aurantia DSM 6220

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: H8L2I2
• Locus tag: FRAAU_RS04855
• Protein ID: WP_014402455.1
• Coordinates: 1072058..1072372 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H8L2I1
• Locus tag: FRAAU_RS04850
• Protein ID: WP_014402454.1
• Coordinates: 1071772..1072077 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRAAU_RS16340	1067201..1068190	+	990	WP_014402446.1	YdaU family protein	-
FRAAU_RS04815	1068177..1068839	+	663	WP_014402447.1	hypothetical protein	-
FRAAU_RS04820	1068836..1069036	+	201	WP_014402448.1	hypothetical protein	-
FRAAU_RS04825	1069033..1069539	+	507	WP_014402449.1	hypothetical protein	-
FRAAU_RS04830	1069536..1069925	+	390	WP_014402450.1	Ref family protein	-
FRAAU_RS04835	1070087..1070419	+	333	WP_083841124.1	DUF1364 domain-containing protein	-
FRAAU_RS04840	1070422..1070799	+	378	WP_014402452.1	VRR-NUC domain-containing protein	-
FRAAU_RS04845	1071041..1071658	+	618	WP_156803485.1	hypothetical protein	-
FRAAU_RS04850	1071772..1072077	-	306	WP_014402454.1	putative addiction module antidote protein	Antitoxin
FRAAU_RS04855	1072058..1072372	-	315	WP_014402455.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FRAAU_RS04860	1072433..1072939	+	507	WP_014402456.1	Rha family transcriptional regulator	-
FRAAU_RS04865	1073003..1073221	+	219	WP_014402457.1	hypothetical protein	-
FRAAU_RS04870	1073205..1073681	+	477	WP_014402458.1	lysozyme	-
FRAAU_RS04875	1073681..1074163	+	483	WP_156803338.1	hypothetical protein	-
FRAAU_RS04880	1074220..1074501	+	282	WP_156803339.1	hypothetical protein	-
FRAAU_RS04885	1074715..1075245	+	531	WP_014402461.1	terminase small subunit	-
FRAAU_RS04890	1075220..1077124	+	1905	WP_014402462.1	phage terminase large subunit family protein	-
FRAAU_RS04895	1077125..1077361	+	237	WP_014402463.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1054919..1236290	181371

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (31-92)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11575.15 Da
• Isoelectric Point: 9.7704

>T26174 WP_014402455.1 NC_017033:c1072372-1072058 [Frateuria aurantia DSM 6220]
MNYIVKQTDGFRSWIASVKDLKAKIAVARRIERASQGNFGDHKPVGDGVSELRIPTGPGYRVYYTIRNLQIVILLCGGDK
SSQSADINKAKQLAKEIQNDHETD

Nucleotide

• Length: 315 bp

>T26174 NC_017033:c1072372-1072058 [Frateuria aurantia DSM 6220]
ATGAACTACATCGTCAAGCAGACCGATGGCTTCCGTAGCTGGATCGCTTCGGTCAAAGACCTCAAGGCGAAGATCGCTGT
TGCTCGGCGCATCGAACGTGCTTCACAGGGCAACTTCGGTGATCACAAGCCTGTTGGCGACGGTGTGTCAGAGCTGCGGA
TCCCAACAGGCCCTGGCTACCGGGTGTACTACACGATTCGCAACCTTCAGATCGTCATTCTGCTCTGCGGTGGCGACAAA
AGTAGCCAAAGCGCAGATATCAACAAGGCCAAGCAGTTGGCCAAGGAGATCCAAAATGACCACGAAACTGACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11032.60 Da
• Isoelectric Point: 5.0916

>AT26174 WP_014402454.1 NC_017033:c1072077-1071772 [Frateuria aurantia DSM 6220]
MTTKLTDFDLSSLLDSEEAITEYLNQVMVDGDQDELLRAFSHVAKAQGMSEIARKSGLGRESLYKALTPGSKPRFETMAR
ISSALGLAMQFTVSPMKAATQ

Nucleotide

• Length: 306 bp

>AT26174 NC_017033:c1072077-1071772 [Frateuria aurantia DSM 6220]
ATGACCACGAAACTGACTGACTTCGATCTCTCCAGCTTGCTCGACAGCGAAGAAGCGATTACCGAATACCTCAACCAGGT
GATGGTCGATGGTGATCAGGACGAACTGCTCCGCGCCTTCAGCCATGTTGCCAAGGCGCAGGGTATGAGCGAGATTGCCC
GCAAGTCAGGCCTTGGGCGGGAAAGCCTTTACAAAGCACTGACTCCCGGCAGCAAGCCGCGATTCGAAACGATGGCCCGG
ATCAGCAGTGCCTTGGGTCTGGCGATGCAATTCACCGTGTCGCCAATGAAGGCTGCGACGCAGTAA

Structures

Toxin

• AlphaFold DB: H8L2I2

Antitoxin

• AlphaFold DB: H8L2I1