Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 366609..367246 Replicon chromosome
Accession NZ_CP107557
Organism Bacillus velezensis strain CPA1-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag OGL87_RS01865 Protein ID WP_003156187.1
Coordinates 366609..366959 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag OGL87_RS01870 Protein ID WP_003156188.1
Coordinates 366965..367246 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OGL87_RS01825 (OGL87_01825) 361649..362251 - 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -
OGL87_RS01830 (OGL87_01830) 362251..363039 - 789 WP_032873003.1 RNA polymerase sigma factor SigB -
OGL87_RS01835 (OGL87_01835) 363005..363487 - 483 WP_007609591.1 anti-sigma B factor RsbW -
OGL87_RS01840 (OGL87_01840) 363484..363813 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
OGL87_RS01845 (OGL87_01845) 363877..364884 - 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
OGL87_RS01850 (OGL87_01850) 364896..365297 - 402 WP_003156178.1 anti-sigma regulatory factor -
OGL87_RS01855 (OGL87_01855) 365300..365665 - 366 WP_003156180.1 RsbT antagonist protein RsbS -
OGL87_RS01860 (OGL87_01860) 365670..366491 - 822 WP_003156182.1 STAS domain-containing protein -
OGL87_RS01865 (OGL87_01865) 366609..366959 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
OGL87_RS01870 (OGL87_01870) 366965..367246 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
OGL87_RS01875 (OGL87_01875) 367366..368535 - 1170 WP_032873001.1 alanine racemase -
OGL87_RS01880 (OGL87_01880) 368652..369659 - 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
OGL87_RS01885 (OGL87_01885) 369824..370189 - 366 WP_007609580.1 holo-ACP synthase -
OGL87_RS01890 (OGL87_01890) 370282..370881 + 600 WP_032872997.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T261735 WP_003156187.1 NZ_CP107557:c366959-366609 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT261735 WP_003156188.1 NZ_CP107557:c367246-366965 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References