Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 489186..489823 Replicon chromosome
Accession NZ_CP107556
Organism Bacillus velezensis strain Bacillus velezensis F3A

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag OF857_RS02535 Protein ID WP_003156187.1
Coordinates 489473..489823 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag OF857_RS02530 Protein ID WP_003156188.1
Coordinates 489186..489467 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OF857_RS02510 (OF857_02510) 485551..486150 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
OF857_RS02515 (OF857_02515) 486243..486608 + 366 WP_070081400.1 holo-ACP synthase -
OF857_RS02520 (OF857_02520) 486773..487780 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
OF857_RS02525 (OF857_02525) 487897..489066 + 1170 WP_128574472.1 alanine racemase -
OF857_RS02530 (OF857_02530) 489186..489467 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
OF857_RS02535 (OF857_02535) 489473..489823 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
OF857_RS02540 (OF857_02540) 489941..490762 + 822 WP_003156182.1 STAS domain-containing protein -
OF857_RS02545 (OF857_02545) 490767..491132 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
OF857_RS02550 (OF857_02550) 491135..491536 + 402 WP_003156178.1 anti-sigma regulatory factor -
OF857_RS02555 (OF857_02555) 491548..492555 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
OF857_RS02560 (OF857_02560) 492618..492947 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
OF857_RS02565 (OF857_02565) 492944..493426 + 483 WP_003156173.1 anti-sigma B factor RsbW -
OF857_RS02570 (OF857_02570) 493392..494180 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
OF857_RS02575 (OF857_02575) 494180..494782 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T261733 WP_003156187.1 NZ_CP107556:489473-489823 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT261733 WP_003156188.1 NZ_CP107556:489186-489467 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References