Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2428421..2429072 Replicon chromosome
Accession NZ_CP107523
Organism Lacticaseibacillus chiayiensis strain AACE 3

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag OFW50_RS11765 Protein ID WP_129301156.1
Coordinates 2428421..2428804 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0R1EMB2
Locus tag OFW50_RS11770 Protein ID WP_010491246.1
Coordinates 2428824..2429072 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OFW50_RS11740 (OFW50_11740) 2423945..2425195 - 1251 WP_213224100.1 RNA-guided endonuclease TnpB family protein -
OFW50_RS11745 (OFW50_11745) 2425270..2425719 + 450 WP_129302982.1 IS200/IS605 family transposase -
OFW50_RS11750 (OFW50_11750) 2425963..2426661 + 699 WP_129302926.1 L,D-transpeptidase -
OFW50_RS11755 (OFW50_11755) 2426736..2427629 - 894 WP_129302966.1 IS3 family transposase -
OFW50_RS11760 (OFW50_11760) 2427626..2428303 - 678 WP_129302967.1 helix-turn-helix domain-containing protein -
OFW50_RS11765 (OFW50_11765) 2428421..2428804 - 384 WP_129301156.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OFW50_RS11770 (OFW50_11770) 2428824..2429072 - 249 WP_010491246.1 antitoxin Antitoxin
OFW50_RS11775 (OFW50_11775) 2429171..2430310 - 1140 WP_129301157.1 alanine racemase -
OFW50_RS11780 (OFW50_11780) 2430297..2430671 - 375 WP_049170363.1 holo-ACP synthase -
OFW50_RS11785 (OFW50_11785) 2430913..2432424 - 1512 WP_129301158.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13784.06 Da        Isoelectric Point: 10.9764

>T261713 WP_129301156.1 NZ_CP107523:c2428804-2428421 [Lacticaseibacillus chiayiensis]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVNLPKPKTYNKN

Download         Length: 384 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8971.07 Da        Isoelectric Point: 4.6061

>AT261713 WP_010491246.1 NZ_CP107523:c2429072-2428824 [Lacticaseibacillus chiayiensis]
MKKQTSVTVTFDAAIKARLDAYCELNDTDTQDVITTAVAKFLQSQAPDINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0R1EMB2

References