Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 91071..91714 Replicon plasmid unnamed2
Accession NZ_CP107520
Organism Klebsiella pneumoniae strain CRKP_1

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag OH528_RS28660 Protein ID WP_001044770.1
Coordinates 91071..91487 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag OH528_RS28665 Protein ID WP_001261282.1
Coordinates 91484..91714 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OH528_RS28640 (87174) 87174..87446 - 273 Protein_108 transposase -
OH528_RS28650 (88428) 88428..89450 - 1023 WP_000361404.1 helicase UvrD -
OH528_RS28655 (89435) 89435..90997 - 1563 WP_004206609.1 AAA family ATPase -
OH528_RS28660 (91071) 91071..91487 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
OH528_RS28665 (91484) 91484..91714 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
OH528_RS28670 (91671) 91671..92132 + 462 WP_014343465.1 hypothetical protein -
OH528_RS28675 (92293) 92293..93237 + 945 WP_011977810.1 hypothetical protein -
OH528_RS28680 (93274) 93274..93666 + 393 WP_011977811.1 hypothetical protein -
OH528_RS28685 (93724) 93724..94245 + 522 WP_013214008.1 hypothetical protein -
OH528_RS28690 (94291) 94291..94494 + 204 WP_011977813.1 hypothetical protein -
OH528_RS28695 (94524) 94524..95528 + 1005 WP_011977814.1 hypothetical protein -
OH528_RS28700 (95712) 95712..96491 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid rmtB / blaTEM-1B / blaCTX-M-65 / blaKPC-2 - 1..154725 154725


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T261710 WP_001044770.1 NZ_CP107520:c91487-91071 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT261710 WP_001261282.1 NZ_CP107520:c91714-91484 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References