Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 91065..91708 Replicon plasmid unnamed2
Accession NZ_CP107470
Organism Klebsiella pneumoniae strain CRKP_11

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag OH523_RS28635 Protein ID WP_001044770.1
Coordinates 91065..91481 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag OH523_RS28640 Protein ID WP_001261282.1
Coordinates 91478..91708 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OH523_RS28615 (87168) 87168..87440 - 273 Protein_108 transposase -
OH523_RS28625 (88422) 88422..89444 - 1023 WP_000361404.1 helicase UvrD -
OH523_RS28630 (89429) 89429..90991 - 1563 WP_004206609.1 AAA family ATPase -
OH523_RS28635 (91065) 91065..91481 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
OH523_RS28640 (91478) 91478..91708 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
OH523_RS28645 (91665) 91665..92126 + 462 WP_014343465.1 hypothetical protein -
OH523_RS28650 (92287) 92287..93231 + 945 WP_011977810.1 hypothetical protein -
OH523_RS28655 (93268) 93268..93660 + 393 WP_011977811.1 hypothetical protein -
OH523_RS28660 (93718) 93718..94239 + 522 WP_013214008.1 hypothetical protein -
OH523_RS28665 (94285) 94285..94488 + 204 WP_011977813.1 hypothetical protein -
OH523_RS28670 (94518) 94518..95522 + 1005 WP_011977814.1 hypothetical protein -
OH523_RS28675 (95706) 95706..96485 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid rmtB / blaTEM-1B / blaCTX-M-65 / blaKPC-2 - 1..154719 154719


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T261453 WP_001044770.1 NZ_CP107470:c91481-91065 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT261453 WP_001261282.1 NZ_CP107470:c91708-91478 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References