Detailed information of TA system TA026140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 4118115..4118759
• Replicon: chromosome
• Accession: NC_016943
• Organism: Blastococcus saxobsidens DD2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H6RLZ3
• Locus tag: BLASA_RS19435
• Protein ID: WP_014377944.1
• Coordinates: 4118343..4118759 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H6RLZ2
• Locus tag: BLASA_RS19430
• Protein ID: WP_014377943.1
• Coordinates: 4118115..4118336 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLASA_RS19405	4114208..4114516	-	309	WP_014377938.1	co-chaperone GroES	-
BLASA_RS19410	4114712..4115938	+	1227	WP_014377939.1	RNA methyltransferase	-
BLASA_RS19415	4115928..4116965	-	1038	WP_014377940.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD	-
BLASA_RS19420	4116962..4117387	-	426	WP_014377941.1	ribosomal protein S18-alanine N-acetyltransferase	-
BLASA_RS19425	4117402..4118037	-	636	WP_014377942.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
BLASA_RS19430	4118115..4118336	+	222	WP_014377943.1	hypothetical protein	Antitoxin
BLASA_RS19435	4118343..4118759	+	417	WP_014377944.1	PIN domain-containing protein	Toxin
BLASA_RS19440	4118785..4119219	-	435	WP_197536360.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
BLASA_RS19445	4119231..4119878	-	648	WP_166486598.1	uracil-DNA glycosylase	-
BLASA_RS19450	4119888..4121081	-	1194	WP_014377947.1	alpha/beta hydrolase	-
BLASA_RS19455	4121081..4122217	-	1137	WP_014377948.1	alanine racemase	-
BLASA_RS19460	4122214..4123680	-	1467	WP_014377949.1	NAD(P)H-hydrate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (3-136)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14656.71 Da
• Isoelectric Point: 7.5234

>T26140 WP_014377944.1 NC_016943:4118343-4118759 [Blastococcus saxobsidens DD2]
VGLLDVNALIALAWDSHVHHVAIRNWFAAHRSSGWATCPITESGFVRISANPTVLPSPLAPATARDVLAGLRSAGAHRFL
VDDVSIVDTDVPATSGHRQVTDAHLLTLARRRGVRLVTFDAGLASLAGNRDVELLTAL

Nucleotide

• Length: 417 bp

>T26140 NC_016943:4118343-4118759 [Blastococcus saxobsidens DD2]
GTGGGACTCCTCGACGTCAACGCGCTGATCGCGCTGGCGTGGGACTCGCACGTCCACCATGTCGCGATCCGGAACTGGTT
CGCCGCCCACCGATCGTCGGGCTGGGCCACCTGCCCGATCACCGAGAGCGGCTTCGTGCGGATCTCCGCCAACCCCACGG
TGCTGCCCAGCCCGCTGGCTCCTGCTACCGCTCGTGACGTCCTCGCCGGACTGCGCAGTGCAGGCGCCCACCGGTTCCTG
GTCGACGACGTATCCATCGTCGATACCGACGTGCCGGCGACGAGCGGGCACCGCCAGGTGACCGACGCGCATCTGCTGAC
GCTCGCCCGCCGCCGAGGCGTCCGGCTGGTCACCTTCGACGCGGGGCTCGCCTCGCTGGCCGGCAACCGGGACGTCGAGC
TGCTCACCGCCCTGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 7606.64 Da
• Isoelectric Point: 4.5203

>AT26140 WP_014377943.1 NC_016943:4118115-4118336 [Blastococcus saxobsidens DD2]
VRTTLDIDDDVVAAARELAAGERRSLGAVISELARRGLTPARVESEDGLPVIRVPSGTAPITPGMVARALEEA

Nucleotide

• Length: 222 bp

>AT26140 NC_016943:4118115-4118336 [Blastococcus saxobsidens DD2]
GTGCGCACCACCCTGGACATCGATGACGACGTCGTCGCTGCGGCTCGCGAGCTCGCCGCTGGTGAGCGCCGCTCTCTCGG
TGCGGTCATCTCCGAGCTCGCCCGCCGAGGGCTGACGCCGGCCCGGGTGGAGAGCGAGGACGGGCTACCGGTGATCCGCG
TGCCGAGCGGGACTGCCCCCATCACCCCGGGGATGGTGGCCCGGGCCCTCGAGGAGGCCTGA

Structures

Toxin

• AlphaFold DB: H6RLZ3

Antitoxin

• AlphaFold DB: H6RLZ2