Detailed information of TA system TA261380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 880..1354
• Replicon: plasmid unnamed5
• Accession: NZ_CP107457
• Organism: Klebsiella pneumoniae strain CRKP_14

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: OH499_RS29020
• Protein ID: WP_004197762.1
• Coordinates: 1088..1354 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: OH499_RS29015
• Protein ID: WP_000879771.1
• Coordinates: 880..1104 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OH499_RS29005	1..213	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
OH499_RS29010	589..717	-	129	WP_000523570.1	hypothetical protein	-
OH499_RS29015	880..1104	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
OH499_RS29020	1088..1354	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OH499_RS29025	1424..1774	-	351	WP_004197756.1	hypothetical protein	-
OH499_RS29030	1840..2385	-	546	WP_023317533.1	hypothetical protein	-
OH499_RS29035	2411..2737	-	327	WP_050516389.1	hypothetical protein	-
OH499_RS29040	3893..4069	+	177	WP_004197757.1	hypothetical protein	-
OH499_RS29045	4059..4403	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
OH499_RS29050	4612..5046	+	435	Protein_9	relaxase/mobilization nuclease domain-containing protein	-
OH499_RS29055	5081..5590	+	510	WP_032433972.1	MbeB family mobilization protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T261380 WP_004197762.1 NZ_CP107457:1088-1354 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T261380 NZ_CP107457:1088-1354 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT261380 WP_000879771.1 NZ_CP107457:880-1104 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT261380 NZ_CP107457:880-1104 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5