Detailed information of TA system TA026138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3399413..3399948
• Replicon: chromosome
• Accession: NC_016943
• Organism: Blastococcus saxobsidens DD2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H6RTY0
• Locus tag: BLASA_RS16090
• Protein ID: WP_014377269.1
• Coordinates: 3399649..3399948 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H6RTX9
• Locus tag: BLASA_RS16085
• Protein ID: WP_014377268.1
• Coordinates: 3399413..3399649 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLASA_RS16070	3394675..3396444	+	1770	WP_014377265.1	hypothetical protein	-
BLASA_RS16075	3396441..3397763	+	1323	WP_014377266.1	helicase-associated domain-containing protein	-
BLASA_RS16080	3397890..3399353	+	1464	WP_197536231.1	DUF3375 domain-containing protein	-
BLASA_RS16085	3399413..3399649	+	237	WP_014377268.1	hypothetical protein	Antitoxin
BLASA_RS16090	3399649..3399948	+	300	WP_014377269.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BLASA_RS16095	3400075..3400722	+	648	WP_014377270.1	DUF4194 domain-containing protein	-
BLASA_RS16100	3400719..3404102	+	3384	WP_014377271.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10700.37 Da
• Isoelectric Point: 8.1023

>T26138 WP_014377269.1 NC_016943:3399649-3399948 [Blastococcus saxobsidens DD2]
MRPIHVARLDKGRPVLVLTRELVRPHLSRVSVAPITNTIRGLSTEVPVGAANGLSHPSVVSCDNVTTVPRTALGRQIGFL
LPDQEEALTEAIHAAFDLY

Nucleotide

• Length: 300 bp

>T26138 NC_016943:3399649-3399948 [Blastococcus saxobsidens DD2]
ATGCGGCCGATCCACGTCGCCCGCCTGGACAAGGGTCGTCCGGTGCTCGTGCTCACCCGGGAACTGGTCCGGCCGCACCT
GTCCCGCGTGAGCGTGGCGCCGATCACGAACACGATCCGCGGTCTGTCGACGGAGGTGCCAGTCGGCGCGGCCAACGGGC
TGAGCCACCCGAGCGTCGTGAGCTGCGACAACGTGACCACAGTGCCGCGCACGGCGCTCGGCCGCCAGATCGGCTTCCTG
CTGCCGGACCAGGAAGAAGCGCTGACCGAAGCGATCCATGCTGCCTTCGATCTCTACTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8577.61 Da
• Isoelectric Point: 4.2667

>AT26138 WP_014377268.1 NC_016943:3399413-3399649 [Blastococcus saxobsidens DD2]
MSKQIAVRLADDLVDFVDTLVAHGRATSRAAVVTRALERERRREVAARDVEILAADPDDPDLDALADFAATVRMDDLD

Nucleotide

• Length: 237 bp

>AT26138 NC_016943:3399413-3399649 [Blastococcus saxobsidens DD2]
ATGAGCAAGCAGATCGCCGTACGCCTGGCAGACGACCTCGTCGACTTCGTCGACACTCTCGTCGCGCACGGACGGGCGAC
CAGCCGCGCTGCGGTAGTGACTCGTGCACTCGAGCGTGAGCGGCGCAGGGAAGTGGCGGCGCGGGATGTCGAGATCCTCG
CCGCCGATCCCGATGATCCTGACCTCGACGCCCTCGCCGACTTCGCCGCAACCGTCCGGATGGATGACCTGGACTGA

Structures

Toxin

• AlphaFold DB: H6RTY0

Antitoxin

• AlphaFold DB: H6RTX9