Detailed information of TA system TA026135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-VapB
• Location: 1354518..1355080
• Replicon: chromosome
• Accession: NC_016943
• Organism: Blastococcus saxobsidens DD2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H6RJP4
• Locus tag: BLASA_RS06430
• Protein ID: WP_014375246.1
• Coordinates: 1354518..1354847 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H6RJP5
• Locus tag: BLASA_RS06435
• Protein ID: WP_014375247.1
• Coordinates: 1354844..1355080 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLASA_RS06410	1350133..1351212	+	1080	WP_014375241.1	LLM class flavin-dependent oxidoreductase	-
BLASA_RS06415	1351288..1351812	+	525	WP_014375242.1	flavin reductase family protein	-
BLASA_RS06420	1351874..1353022	+	1149	WP_166486487.1	alpha/beta hydrolase	-
BLASA_RS06425	1353133..1353924	+	792	WP_166486488.1	alpha/beta fold hydrolase	-
BLASA_RS06430	1354518..1354847	-	330	WP_014375246.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BLASA_RS06435	1354844..1355080	-	237	WP_014375247.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BLASA_RS06440	1355250..1355906	-	657	WP_014375248.1	GntR family transcriptional regulator	-
BLASA_RS06445	1355988..1356422	+	435	WP_014375249.1	nuclear transport factor 2 family protein	-
BLASA_RS06450	1356419..1357174	+	756	WP_014375250.1	alpha/beta hydrolase	-
BLASA_RS06455	1357171..1357827	+	657	WP_083877948.1	carbon-nitrogen hydrolase	-
BLASA_RS06460	1357877..1359184	+	1308	WP_014374930.1	IS256-like element ISBsa1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1332859..1467218	134359
	-	flank	IS/Tn	-	-	1357877..1359184	1307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (9-46)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11915.94 Da
• Isoelectric Point: 7.5789

>T26135 WP_014375246.1 NC_016943:c1354847-1354518 [Blastococcus saxobsidens DD2]
VTTLPARGEVWWCEMAEIGRRPVVVLSRDAVIPRHRRALVAPCTTTIRGLVSEVRLEPGEDPVPRRCVVNMDSVESVSVA
VLVERLGRLADARMRQICGALAVAVDCAD

Nucleotide

• Length: 330 bp

>T26135 NC_016943:c1354847-1354518 [Blastococcus saxobsidens DD2]
GTGACTACGCTGCCCGCGCGGGGCGAGGTCTGGTGGTGCGAGATGGCCGAGATCGGGCGCCGCCCGGTCGTCGTCCTCTC
CCGCGACGCCGTGATCCCCCGACACCGGCGCGCACTCGTCGCACCATGCACCACCACCATCCGCGGACTCGTCAGCGAAG
TCAGGCTCGAGCCCGGCGAGGACCCGGTACCCCGCCGCTGCGTGGTCAACATGGACTCGGTCGAGAGCGTCTCTGTCGCG
GTCCTCGTTGAGCGGCTCGGCCGGTTGGCCGACGCCCGCATGCGGCAGATCTGCGGAGCCCTCGCCGTCGCGGTCGACTG
TGCGGACTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8735.55 Da
• Isoelectric Point: 4.3943

>AT26135 WP_014375247.1 NC_016943:c1355080-1354844 [Blastococcus saxobsidens DD2]
MSRTRLSTTVDEDLLQAARRVRVGDTDAAMIDEALRALVARHRSAEVEASYTAYDEHPLDEPDEWGDLASFREATARS

Nucleotide

• Length: 237 bp

>AT26135 NC_016943:c1355080-1354844 [Blastococcus saxobsidens DD2]
ATGAGTAGAACACGGCTCAGCACTACGGTCGACGAAGATCTGCTCCAAGCCGCTCGACGTGTGCGAGTCGGTGACACCGA
CGCCGCCATGATCGATGAGGCGCTCCGCGCGCTGGTGGCGCGCCACCGTTCAGCCGAAGTCGAGGCGAGCTACACCGCCT
ACGACGAACACCCGCTCGACGAACCAGACGAGTGGGGTGACCTGGCGTCGTTCCGCGAAGCCACCGCACGGTCGTGA

Structures

Toxin

• AlphaFold DB: H6RJP4

Antitoxin

• AlphaFold DB: H6RJP5