Detailed information of TA system TA026131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 322652..323322
• Replicon: chromosome
• Accession: NC_016943
• Organism: Blastococcus saxobsidens DD2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H6RM60
• Locus tag: BLASA_RS01470
• Protein ID: WP_014374219.1
• Coordinates: 322652..323062 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H6RM61
• Locus tag: BLASA_RS01475
• Protein ID: WP_014374220.1
• Coordinates: 323059..323322 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLASA_RS01445	319551..319895	-	345	WP_041775541.1	type II toxin-antitoxin system PemK/MazF family toxin	-
BLASA_RS01450	319889..320137	-	249	WP_041775983.1	ribbon-helix-helix protein, CopG family	-
BLASA_RS01455	320183..321244	+	1062	WP_014374216.1	4Fe-4S dicluster domain-containing protein	-
BLASA_RS01460	321247..322308	+	1062	WP_014374217.1	polysulfide reductase NrfD	-
BLASA_RS01465	322423..322626	+	204	WP_014374218.1	hypothetical protein	-
BLASA_RS01470	322652..323062	-	411	WP_014374219.1	type II toxin-antitoxin system VapC family toxin	Toxin
BLASA_RS01475	323059..323322	-	264	WP_014374220.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BLASA_RS01480	323470..323871	-	402	WP_014374221.1	ribonuclease	-
BLASA_RS01485	323868..324095	-	228	WP_014374222.1	hypothetical protein	-
BLASA_RS01490	324487..324690	+	204	WP_014374223.1	hypothetical protein	-
BLASA_RS01495	324760..325161	-	402	WP_014374224.1	type II toxin-antitoxin system VapC family toxin	-
BLASA_RS01500	325158..325424	-	267	WP_014374225.1	ribbon-helix-helix protein, CopG family	-
BLASA_RS01505	325508..326305	-	798	WP_014374226.1	hypothetical protein	-
BLASA_RS01510	326307..326696	-	390	WP_014374227.1	S-adenosylmethionine decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	294300..382307	88007

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (1-86)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14356.98 Da
• Isoelectric Point: 4.3360

>T26131 WP_014374219.1 NC_016943:c323062-322652 [Blastococcus saxobsidens DD2]
MIVDTSAVVAILRGEPDADRYIQALATADEARTSAATYLETAVVVDANRDPVLSGRFDDLVAAVPITVEPVTRSHADIAR
RAYRDFGKGSGHAARLNFGDCFSYALARATGEPLLFTGTDFSATDVTPALDPADPA

Nucleotide

• Length: 411 bp

>T26131 NC_016943:c323062-322652 [Blastococcus saxobsidens DD2]
ATGATCGTCGACACGTCCGCTGTCGTCGCGATCCTGCGCGGAGAACCGGACGCCGACCGCTACATCCAGGCACTGGCCAC
CGCGGACGAGGCTCGCACCTCGGCGGCGACCTACCTGGAGACCGCTGTCGTGGTGGACGCCAACCGTGACCCGGTGCTCA
GTGGCCGCTTCGACGACCTCGTGGCCGCGGTGCCCATCACCGTCGAACCGGTCACGCGGTCGCATGCGGACATCGCCCGC
CGGGCCTATCGCGACTTCGGCAAGGGCAGCGGCCACGCCGCCCGCCTGAACTTCGGCGACTGCTTCAGCTACGCGCTGGC
CCGCGCCACGGGCGAACCACTGCTCTTCACGGGCACCGACTTCTCGGCGACCGATGTCACCCCGGCCCTCGATCCGGCGG
ATCCTGCCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9673.77 Da
• Isoelectric Point: 5.1409

>AT26131 WP_014374220.1 NC_016943:c323322-323059 [Blastococcus saxobsidens DD2]
MGLNIKNPETEELARQLADATGENLTRAITVAVRERLDRIQRRGTAAAADRTALLQRIAGDAADRWVEPYRSSDHGDLLY
DETGLPR

Nucleotide

• Length: 264 bp

>AT26131 NC_016943:c323322-323059 [Blastococcus saxobsidens DD2]
ATGGGCCTCAACATCAAGAACCCGGAAACCGAGGAGCTCGCCCGCCAACTCGCCGACGCAACCGGCGAGAACCTCACGCG
GGCCATCACCGTGGCGGTGCGCGAACGTCTCGATCGGATTCAACGCCGGGGCACCGCCGCGGCGGCCGACCGCACCGCCC
TGCTCCAGAGGATTGCCGGGGATGCAGCCGATCGGTGGGTCGAGCCCTATCGCAGCAGCGACCACGGCGACCTGCTCTAC
GACGAGACCGGCCTGCCCCGATGA

Structures

Toxin

• AlphaFold DB: H6RM60

Antitoxin

• AlphaFold DB: H6RM61