Detailed information of TA system TA026130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 319551..320137
• Replicon: chromosome
• Accession: NC_016943
• Organism: Blastococcus saxobsidens DD2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BLASA_RS01445
• Protein ID: WP_041775541.1
• Coordinates: 319551..319895 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BLASA_RS01450
• Protein ID: WP_041775983.1
• Coordinates: 319889..320137 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLASA_RS01420	314585..314851	+	267	WP_166486438.1	hypothetical protein	-
BLASA_RS01425	315157..315378	+	222	WP_014374209.1	hypothetical protein	-
BLASA_RS01430	315469..315843	-	375	WP_014374210.1	type II toxin-antitoxin system PemK/MazF family toxin	-
BLASA_RS24720	315840..316073	-	234	WP_014374211.1	hypothetical protein	-
BLASA_RS01435	316051..316833	+	783	WP_166486439.1	dehydrogenase	-
BLASA_RS01440	316846..319548	+	2703	WP_014374213.1	molybdopterin-dependent oxidoreductase	-
BLASA_RS01445	319551..319895	-	345	WP_041775541.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BLASA_RS01450	319889..320137	-	249	WP_041775983.1	ribbon-helix-helix protein, CopG family	Antitoxin
BLASA_RS01455	320183..321244	+	1062	WP_014374216.1	4Fe-4S dicluster domain-containing protein	-
BLASA_RS01460	321247..322308	+	1062	WP_014374217.1	polysulfide reductase NrfD	-
BLASA_RS01465	322423..322626	+	204	WP_014374218.1	hypothetical protein	-
BLASA_RS01470	322652..323062	-	411	WP_014374219.1	type II toxin-antitoxin system VapC family toxin	-
BLASA_RS01475	323059..323322	-	264	WP_014374220.1	type II toxin-antitoxin system VapB family antitoxin	-
BLASA_RS01480	323470..323871	-	402	WP_014374221.1	ribonuclease	-
BLASA_RS01485	323868..324095	-	228	WP_014374222.1	hypothetical protein	-
BLASA_RS01490	324487..324690	+	204	WP_014374223.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	294300..382307	88007

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-114)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-39)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12338.34 Da
• Isoelectric Point: 6.4642

>T26130 WP_041775541.1 NC_016943:c319895-319551 [Blastococcus saxobsidens DD2]
VVIQRGSIHWADLGEPRRSEPGWRRPIVVIQIDRYTESRLATVLAAIVTGNERLGAVPGNVVIEPHDSGLPRTSVINVTA
LVAVDKAFIEPAIGLLPLAVMQQVDDGLRFVLGL

Nucleotide

• Length: 345 bp

>T26130 NC_016943:c319895-319551 [Blastococcus saxobsidens DD2]
GTGGTGATCCAGCGAGGCAGCATCCACTGGGCAGACCTCGGCGAACCTCGGCGTAGCGAACCCGGCTGGCGACGGCCGAT
CGTCGTCATTCAGATCGATCGCTACACCGAGAGCCGCCTGGCGACCGTTCTTGCGGCCATCGTGACCGGGAATGAGCGGC
TCGGTGCCGTGCCGGGCAACGTCGTCATCGAGCCGCACGACAGCGGACTTCCCCGCACGTCGGTGATCAACGTGACCGCG
CTGGTGGCCGTGGACAAGGCCTTTATCGAGCCGGCCATCGGTCTCCTGCCCCTGGCCGTCATGCAACAGGTCGACGATGG
GTTGCGTTTCGTCCTGGGCCTCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9166.08 Da
• Isoelectric Point: 4.3712

>AT26130 WP_041775983.1 NC_016943:c320137-319889 [Blastococcus saxobsidens DD2]
VKTAISVPDHILEQVNRKARDLGLNRSQFFSQAAERYLREIDDDITERINAALDAAGDDDSNAVAAAHGRRMLLELTADD
EW

Nucleotide

• Length: 249 bp

>AT26130 NC_016943:c320137-319889 [Blastococcus saxobsidens DD2]
GTGAAGACAGCGATCTCGGTTCCAGACCACATCCTCGAGCAGGTGAACCGGAAGGCCAGAGACCTCGGCCTGAACCGCTC
CCAGTTCTTCAGCCAGGCGGCCGAGCGATACCTGCGCGAAATCGACGACGACATCACCGAGCGCATCAACGCGGCACTGG
ATGCCGCGGGTGACGACGACAGCAACGCGGTCGCCGCCGCGCATGGCCGCCGGATGTTGCTGGAGCTCACTGCCGACGAC
GAGTGGTGA