Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5328789..5329414 Replicon chromosome
Accession NZ_CP107412
Organism Klebsiella pneumoniae strain CRKP_22

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag OH525_RS26280 Protein ID WP_002882817.1
Coordinates 5328789..5329172 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OH525_RS26285 Protein ID WP_004150355.1
Coordinates 5329172..5329414 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OH525_RS26265 5326155..5327057 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
OH525_RS26270 5327054..5327689 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
OH525_RS26275 5327686..5328615 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
OH525_RS26280 5328789..5329172 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
OH525_RS26285 5329172..5329414 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OH525_RS26290 5329619..5330536 + 918 WP_002882812.1 alpha/beta hydrolase -
OH525_RS26295 5330550..5331491 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
OH525_RS26300 5331536..5331973 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
OH525_RS26305 5331970..5332830 - 861 WP_002882807.1 virulence factor BrkB family protein -
OH525_RS26310 5332824..5333423 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T261167 WP_002882817.1 NZ_CP107412:c5329172-5328789 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT261167 WP_004150355.1 NZ_CP107412:c5329414-5329172 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References