Detailed information of TA system TA026112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2115962..2116476
• Replicon: chromosome
• Accession: NC_016928
• Organism: Staphylococcus aureus subsp. aureus M013

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: M013TW_RS10550
• Protein ID: WP_000581792.1
• Coordinates: 2115962..2116171 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A0D1GBN9
• Locus tag: M013TW_RS10555
• Protein ID: WP_000138934.1
• Coordinates: 2116183..2116476 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M013TW_RS10530	2111470..2112828	-	1359	WP_000611468.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
M013TW_RS10535	2112843..2113913	-	1071	WP_000159641.1	D-alanine--D-alanine ligase	-
M013TW_RS10540	2114231..2115433	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
M013TW_RS10545	2115697..2115834	-	138	WP_000828354.1	hypothetical protein	-
M013TW_RS10550	2115962..2116171	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
M013TW_RS10555	2116183..2116476	-	294	WP_000138934.1	copper-sensing transcriptional repressor CsoR	Antitoxin
M013TW_RS10560	2116642..2118126	+	1485	WP_000571549.1	cardiolipin synthase	-
M013TW_RS10565	2118154..2118801	+	648	WP_001187610.1	HD domain-containing protein	-
M013TW_RS10590	2119616..2120488	-	873	WP_000725802.1	membrane protein insertase YidC	-
M013TW_RS10595	2120574..2121215	-	642	WP_000483147.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T26112 WP_000581792.1 NC_016928:c2116171-2115962 [Staphylococcus aureus subsp. aureus M013]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T26112 NC_016928:c2116171-2115962 [Staphylococcus aureus subsp. aureus M013]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAAACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11185.77 Da
• Isoelectric Point: 6.2243

>AT26112 WP_000138934.1 NC_016928:c2116476-2116183 [Staphylococcus aureus subsp. aureus M013]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT26112 NC_016928:c2116476-2116183 [Staphylococcus aureus subsp. aureus M013]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: A0A0D1GBN9