261115

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2655963..2656588 Replicon chromosome
Accession NZ_CP107403
Organism Klebsiella pneumoniae strain CRKP_25

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag OH516_RS13060 Protein ID WP_002882817.1
Coordinates 2655963..2656346 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OH516_RS13065 Protein ID WP_004150355.1
Coordinates 2656346..2656588 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OH516_RS13045 2653329..2654231 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
OH516_RS13050 2654228..2654863 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
OH516_RS13055 2654860..2655789 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
OH516_RS13060 2655963..2656346 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
OH516_RS13065 2656346..2656588 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OH516_RS13070 2656793..2657710 + 918 WP_002882812.1 alpha/beta hydrolase -
OH516_RS13075 2657724..2658665 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
OH516_RS13080 2658710..2659147 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
OH516_RS13085 2659144..2660004 - 861 WP_002882807.1 virulence factor BrkB family protein -
OH516_RS13090 2659998..2660597 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T261115 WP_002882817.1 NZ_CP107403:c2656346-2655963 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT261115 WP_004150355.1 NZ_CP107403:c2656588-2656346 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References