Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2025990..2026504 Replicon chromosome
Accession NC_016912
Organism Staphylococcus aureus subsp. aureus VC40

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag SAVC_RS10145 Protein ID WP_000581792.1
Coordinates 2025990..2026199 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag SAVC_RS10150 Protein ID WP_000138932.1
Coordinates 2026211..2026504 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAVC_RS10125 2021498..2022856 - 1359 WP_000611465.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
SAVC_RS10130 2022871..2023941 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
SAVC_RS10135 2024259..2025461 + 1203 WP_001109939.1 rod shape-determining protein RodA -
SAVC_RS10140 2025725..2025862 - 138 WP_000828354.1 hypothetical protein -
SAVC_RS10145 2025990..2026199 - 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
SAVC_RS10150 2026211..2026504 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
SAVC_RS10155 2026670..2028154 + 1485 WP_000571549.1 cardiolipin synthase -
SAVC_RS10160 2028182..2028829 + 648 WP_001187629.1 HD domain-containing protein -
SAVC_RS10175 2029642..2030514 - 873 WP_000725802.1 membrane protein insertase YidC -
SAVC_RS10180 2030600..2031241 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T26100 WP_000581792.1 NC_016912:c2026199-2025990 [Staphylococcus aureus subsp. aureus VC40]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T26100 NC_016912:c2026199-2025990 [Staphylococcus aureus subsp. aureus VC40]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT26100 WP_000138932.1 NC_016912:c2026504-2026211 [Staphylococcus aureus subsp. aureus VC40]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT26100 NC_016912:c2026504-2026211 [Staphylococcus aureus subsp. aureus VC40]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References