Detailed information of TA system TA260980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 171669..172339
• Replicon: plasmid unnamed1
• Accession: NZ_CP107373
• Organism: Klebsiella pneumoniae strain CRKP_31

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: OH494_RS27885
• Protein ID: WP_004213072.1
• Coordinates: 171896..172339 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: OH494_RS27880
• Protein ID: WP_004213073.1
• Coordinates: 171669..171899 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OH494_RS27855	167892..168791	-	900	WP_004225022.1	nucleotide-binding protein	-
OH494_RS27860	168781..169071	-	291	WP_004213078.1	hypothetical protein	-
OH494_RS27865	169423..169629	+	207	WP_004213077.1	hypothetical protein	-
OH494_RS27870	169619..169912	-	294	WP_004213076.1	hypothetical protein	-
OH494_RS27875	169928..171061	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
OH494_RS27880	171669..171899	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
OH494_RS27885	171896..172339	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
OH494_RS27890	172488..172739	+	252	WP_186987481.1	hypothetical protein	-
OH494_RS27895	172762..173066	-	305	Protein_196	transposase	-
OH494_RS27900	173483..174118	+	636	Protein_197	mucoid phenotype regulator RmpA2	-
OH494_RS27905	174636..175039	-	404	Protein_198	GAF domain-containing protein	-
OH494_RS27910	175130..176050	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
OH494_RS27915	176099..176590	-	492	WP_011251327.1	DM13 domain-containing protein	-
OH494_RS27920	176653..176928	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / rmpA2 / iutA / iucD / iucC / iucB / iucA	1..193835	193835

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T260980 WP_004213072.1 NZ_CP107373:171896-172339 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T260980 NZ_CP107373:171896-172339 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT260980 WP_004213073.1 NZ_CP107373:171669-171899 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT260980 NZ_CP107373:171669-171899 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620