Detailed information of TA system TA026094

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 152684..153222
• Replicon: plasmid p174
• Accession: NC_016907
• Organism: Gordonia polyisoprenivorans VH2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GPOL_RS25935
• Protein ID: WP_043457659.1
• Coordinates: 152890..153222 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A846WSS4
• Locus tag: GPOL_RS25930
• Protein ID: WP_026920098.1
• Coordinates: 152684..152893 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPOL_RS25905	148191..148724	+	534	WP_014362239.1	TetR/AcrR family transcriptional regulator	-
GPOL_RS25910	148842..149525	+	684	WP_014362240.1	haloacid dehalogenase type II	-
GPOL_RS25915	149660..150928	+	1269	WP_014362241.1	APC family permease	-
GPOL_RS25920	150975..151766	+	792	WP_006368694.1	ABC transporter permease	-
GPOL_RS25925	151864..152043	+	180	WP_014362243.1	hypothetical protein	-
GPOL_RS25930	152684..152893	+	210	WP_026920098.1	DUF3018 family protein	Antitoxin
GPOL_RS25935	152890..153222	+	333	WP_043457659.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GPOL_RS25940	153441..153806	+	366	WP_043457662.1	metalloregulator ArsR/SmtB family transcription factor	-
GPOL_RS25945	153803..154891	+	1089	WP_043457664.1	ACR3 family arsenite efflux transporter	-
GPOL_RS25950	154884..155294	+	411	WP_014362247.1	low molecular weight phosphatase family protein	-
GPOL_RS25955	155313..155807	-	495	WP_014362248.1	VOC family protein	-
GPOL_RS25960	155910..156275	+	366	WP_014362249.1	metalloregulator ArsR/SmtB family transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..174494	174494

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• Antitoxin MazE-like (4-67)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12075.97 Da
• Isoelectric Point: 6.3029

>T26094 WP_043457659.1 NC_016907:152890-153222 [Gordonia polyisoprenivorans VH2]
VIRGEIWTVAGGVYASKPRPAVIVQDDLFDSTLSVVVAPITSQLIDAPLLRIRIPCDRDSISGLERDSDVMVDKLTAVRR
SNVQTRVGRLTSDQLVEVERSLMAFLGLAR

Nucleotide

• Length: 333 bp

>T26094 NC_016907:152890-153222 [Gordonia polyisoprenivorans VH2]
GTGATTCGCGGCGAAATCTGGACCGTTGCGGGTGGTGTGTATGCATCCAAGCCGCGGCCGGCCGTCATCGTGCAGGATGA
CCTGTTCGATTCAACTCTGTCGGTGGTAGTTGCTCCGATCACGAGCCAGCTCATCGATGCGCCCCTGTTGCGGATTCGTA
TCCCGTGTGATCGCGATTCGATCTCGGGCCTCGAGCGGGACAGCGACGTGATGGTTGACAAGCTAACCGCGGTACGACGG
TCGAACGTTCAGACGCGTGTCGGTCGTCTGACATCCGACCAACTCGTTGAAGTCGAGCGCTCGTTGATGGCGTTTCTGGG
ACTAGCGCGATGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7925.79 Da
• Isoelectric Point: 4.4550

>AT26094 WP_026920098.1 NC_016907:152684-152893 [Gordonia polyisoprenivorans VH2]
MAVRDRVGEYRRRMRERGMRPLQVWVPDVRTPEFAAQAHRQSVLVAEADADGDEQDFVEAVAAPWDDEA

Nucleotide

• Length: 210 bp

>AT26094 NC_016907:152684-152893 [Gordonia polyisoprenivorans VH2]
ATGGCAGTGAGGGACAGGGTCGGTGAGTACCGACGTCGGATGCGTGAGCGCGGAATGCGCCCGTTGCAGGTCTGGGTTCC
TGACGTACGAACGCCAGAGTTTGCCGCGCAAGCCCACAGGCAATCGGTTCTCGTGGCAGAGGCAGACGCCGATGGGGATG
AGCAGGATTTCGTCGAGGCTGTCGCCGCGCCTTGGGACGACGAGGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A846WSS4