Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2513788..2514413 Replicon chromosome
Accession NZ_CP107352
Organism Klebsiella pneumoniae strain CRKP_35

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag OH515_RS12075 Protein ID WP_002882817.1
Coordinates 2513788..2514171 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OH515_RS12080 Protein ID WP_004150355.1
Coordinates 2514171..2514413 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OH515_RS12060 (2511154) 2511154..2512056 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
OH515_RS12065 (2512053) 2512053..2512688 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
OH515_RS12070 (2512685) 2512685..2513614 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
OH515_RS12075 (2513788) 2513788..2514171 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
OH515_RS12080 (2514171) 2514171..2514413 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OH515_RS12085 (2514618) 2514618..2515535 + 918 WP_004181612.1 alpha/beta hydrolase -
OH515_RS12090 (2515549) 2515549..2516490 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
OH515_RS12095 (2516535) 2516535..2516972 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
OH515_RS12100 (2516969) 2516969..2517829 - 861 WP_002882807.1 virulence factor BrkB family protein -
OH515_RS12105 (2517823) 2517823..2518422 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T260877 WP_002882817.1 NZ_CP107352:c2514171-2513788 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT260877 WP_004150355.1 NZ_CP107352:c2514413-2514171 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References