Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1950488..1951298 Replicon chromosome
Accession NZ_CP107352
Organism Klebsiella pneumoniae strain CRKP_35

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag OH515_RS09435 Protein ID WP_004178461.1
Coordinates 1950488..1951021 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag OH515_RS09440 Protein ID WP_002887278.1
Coordinates 1951032..1951298 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OH515_RS09430 (1949319) 1949319..1950440 + 1122 WP_002887282.1 cupin domain-containing protein -
OH515_RS09435 (1950488) 1950488..1951021 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
OH515_RS09440 (1951032) 1951032..1951298 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
OH515_RS09445 (1951401) 1951401..1952834 - 1434 WP_016946714.1 Cu(+)/Ag(+) sensor histidine kinase -
OH515_RS09450 (1952824) 1952824..1953507 - 684 WP_117113634.1 copper response regulator transcription factor CusR -
OH515_RS09455 (1953680) 1953680..1955065 + 1386 WP_117113633.1 efflux transporter outer membrane subunit -
OH515_RS09460 (1955083) 1955083..1955427 + 345 WP_135696080.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T260876 WP_004178461.1 NZ_CP107352:c1951021-1950488 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT260876 WP_002887278.1 NZ_CP107352:c1951298-1951032 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References