Detailed information of TA system TA260615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 2996932..2998652
• Replicon: chromosome
• Accession: NZ_CP107284
• Organism: Escherichia coli strain SW4848

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A7U9J2A7
• Locus tag: OF379_RS14640
• Protein ID: WP_000312833.1
• Coordinates: 2996932..2997588 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: A0A8E0ML02
• Locus tag: OF379_RS14645
• Protein ID: WP_021523959.1
• Coordinates: 2997585..2998652 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OF379_RS14620 (2992332)	2992332..2992586	-	255	WP_154670580.1	hypothetical protein	-
OF379_RS14625 (2992868)	2992868..2993107	+	240	WP_001431807.1	hypothetical protein	-
OF379_RS14630 (2993147)	2993147..2995585	+	2439	WP_000985465.1	type I restriction-modification system subunit M	-
OF379_RS14635 (2995585)	2995585..2996919	+	1335	WP_083583150.1	restriction endonuclease subunit S	-
OF379_RS14640 (2996932)	2996932..2997588	+	657	WP_000312833.1	toxin-antitoxin system toxin DNA ADP-ribosyl transferase DarT	Toxin
OF379_RS14645 (2997585)	2997585..2998652	+	1068	WP_021523959.1	toxin-antitoxin system antitoxin DNA ADP-ribosyl glycohydrolase DarG	Antitoxin
OF379_RS14650 (2998671)	2998671..3001766	+	3096	WP_087597364.1	HsdR family type I site-specific deoxyribonuclease	-
OF379_RS14655 (3001766)	3001766..3002482	+	717	WP_001122107.1	SprT family zinc-dependent metalloprotease	-
OF379_RS14660 (3002827)	3002827..3003441	+	615	Protein_2873	IS3-like element IS600 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgB / csgD / csgE / csgF / csgG / aafC / aggD / iroC / iroD / iroE / iroN	2964165..3028672	64507

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25233.56 Da
• Isoelectric Point: 6.2145

>T260615 WP_000312833.1 NZ_CP107284:2996932-2997588 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Nucleotide

• Length: 657 bp

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40671.76 Da
• Isoelectric Point: 6.1751

>AT260615 WP_021523959.1 NZ_CP107284:2997585-2998652 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGKCQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

Structures

Toxin

• AlphaFold DB: A0A7U9J2A7