Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 479851..480488 Replicon chromosome
Accession NZ_CP107079
Organism Bacillus velezensis strain LT-2

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag OD347_RS02465 Protein ID WP_003156187.1
Coordinates 480138..480488 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag OD347_RS02460 Protein ID WP_003156188.1
Coordinates 479851..480132 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OD347_RS02440 476217..476816 - 600 WP_088612192.1 rhomboid family intramembrane serine protease -
OD347_RS02445 476909..477274 + 366 WP_088612193.1 holo-ACP synthase -
OD347_RS02450 477439..478446 + 1008 WP_013351094.1 outer membrane lipoprotein carrier protein LolA -
OD347_RS02455 478563..479732 + 1170 WP_065521859.1 alanine racemase -
OD347_RS02460 479851..480132 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
OD347_RS02465 480138..480488 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
OD347_RS02470 480609..481430 + 822 WP_045508380.1 STAS domain-containing protein -
OD347_RS02475 481435..481800 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
OD347_RS02480 481803..482203 + 401 Protein_450 anti-sigma regulatory factor -
OD347_RS02485 482215..483222 + 1008 WP_013351098.1 PP2C family protein-serine/threonine phosphatase -
OD347_RS02490 483286..483615 + 330 WP_014469786.1 anti-sigma factor antagonist -
OD347_RS02495 483612..484094 + 483 WP_003156173.1 anti-sigma B factor RsbW -
OD347_RS02500 484060..484848 + 789 WP_013351101.1 RNA polymerase sigma factor SigB -
OD347_RS02505 484848..485450 + 603 WP_045508359.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T260518 WP_003156187.1 NZ_CP107079:480138-480488 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT260518 WP_003156188.1 NZ_CP107079:479851-480132 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References