Detailed information of TA system TA260477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 162371..163994
• Replicon: plasmid unnamed1
• Accession: NZ_CP107041
• Organism: Klebsiella oxytoca strain TYL-T1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: NQA44_RS28400
• Protein ID: WP_263616043.1
• Coordinates: 162669..163994 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: NQA44_RS28395
• Protein ID: WP_024136327.1
• Coordinates: 162371..162664 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NQA44_RS28365 (NQA44_28365)	158243..159484	+	1242	WP_001053338.1	VWA domain-containing protein	-
NQA44_RS28370 (NQA44_28370)	159575..159706	+	132	WP_000374059.1	hypothetical protein	-
NQA44_RS28375 (NQA44_28375)	160047..160328	-	282	WP_000077926.1	hypothetical protein	-
NQA44_RS28380 (NQA44_28380)	160378..160569	-	192	WP_000398480.1	hypothetical protein	-
NQA44_RS28385 (NQA44_28385)	160661..161002	-	342	WP_001151575.1	hypothetical protein	-
NQA44_RS28390 (NQA44_28390)	161375..161767	+	393	WP_000341066.1	hypothetical protein	-
NQA44_RS28395 (NQA44_28395)	162371..162664	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
NQA44_RS28400 (NQA44_28400)	162669..163994	+	1326	WP_263616043.1	type II toxin-antitoxin system HipA family toxin	Toxin
NQA44_RS28405 (NQA44_28405)	164055..164261	+	207	WP_000134171.1	hypothetical protein	-
NQA44_RS28410 (NQA44_28410)	164363..164773	+	411	WP_000985911.1	hypothetical protein	-
NQA44_RS28415 (NQA44_28415)	164786..165031	+	246	Protein_164	HNH endonuclease	-
NQA44_RS28420 (NQA44_28420)	165084..165788	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
NQA44_RS28425 (NQA44_28425)	165835..166410	-	576	WP_072740834.1	recombinase family protein	-
NQA44_RS28430 (NQA44_28430)	166536..166886	-	351	WP_000454193.1	DUF3330 domain-containing protein	-
NQA44_RS28435 (NQA44_28435)	167089..168102	-	1014	WP_000845048.1	class 1 integron integrase IntI1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	cmlA1 / dfrA14 / ant(3'')-Ia / blaOXA-10 / qnrS1 / tet(A) / blaTEM-1B / aadA2 / sul3 / aac(3)-IVa / aph(4)-Ia / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / lnu(F)	-	1..208719	208719
	-	inside	Integron	lnu(F) / cml / cmlA1 / ARR-3 / ant(3'')-Ia / blaOXA-10 / dfrA14	-	2..208508	208506

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (155-404)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50309.23 Da
• Isoelectric Point: 6.1935

>T260477 WP_263616043.1 NZ_CP107041:162669-163994 [Klebsiella oxytoca]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQYRRLAEK

Nucleotide

• Length: 1326 bp

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT260477 WP_024136327.1 NZ_CP107041:162371-162664 [Klebsiella oxytoca]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

Structures

Antitoxin

• AlphaFold DB: A0A5Z6V2D1