Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2501776..2502412 Replicon chromosome
Accession NZ_CP107039
Organism Bacillus subtilis strain 11060

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ODQ18_RS13025 Protein ID WP_003156187.1
Coordinates 2501776..2502126 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag ODQ18_RS13030 Protein ID WP_003225183.1
Coordinates 2502131..2502412 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ODQ18_RS12985 (2496820) 2496820..2497419 - 600 WP_003246608.1 phosphoserine phosphatase RsbX -
ODQ18_RS12990 (2497419) 2497419..2498207 - 789 WP_003246715.1 RNA polymerase sigma factor SigB -
ODQ18_RS12995 (2498173) 2498173..2498655 - 483 WP_003234299.1 anti-sigma B factor RsbW -
ODQ18_RS13000 (2498652) 2498652..2498981 - 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
ODQ18_RS13005 (2499043) 2499043..2500050 - 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
ODQ18_RS13010 (2500062) 2500062..2500463 - 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
ODQ18_RS13015 (2500467) 2500467..2500832 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
ODQ18_RS13020 (2500837) 2500837..2501661 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
ODQ18_RS13025 (2501776) 2501776..2502126 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ODQ18_RS13030 (2502131) 2502131..2502412 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ODQ18_RS13035 (2502528) 2502528..2503697 - 1170 WP_015252766.1 alanine racemase -
ODQ18_RS13040 (2503811) 2503811..2504827 - 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
ODQ18_RS13045 (2504993) 2504993..2505358 - 366 WP_015252768.1 holo-ACP synthase -
ODQ18_RS13050 (2505453) 2505453..2506052 + 600 WP_041850724.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T260467 WP_003156187.1 NZ_CP107039:c2502126-2501776 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT260467 WP_003225183.1 NZ_CP107039:c2502412-2502131 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References