Detailed information of TA system TA026032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 6002..6645
• Replicon: plasmid pKPHS2
• Accession: NC_016846
• Organism: Klebsiella pneumoniae subsp. pneumoniae HS11286

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A0H3GYM2
• Locus tag: KPHS_p200130
• Protein ID: YP_005229630.1
• Coordinates: 6229..6645 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A0H3GZG3
• Locus tag: KPHS_p200120
• Protein ID: YP_005229629.1
• Coordinates: 6002..6232 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPHS_p200040	1225..2004	-	780	YP_005229620.1	plasmid F resolvase-like protein	-
KPHS_p200050	2013..2144	-	132	YP_005229621.1	hypothetical protein	-
KPHS_p200060	2188..3192	-	1005	YP_005229622.1	hypothetical protein	-
KPHS_p200070	3222..3425	-	204	YP_005229623.1	hypothetical protein	-
KPHS_p200080	3471..3992	-	522	YP_005229624.1	hypothetical protein	-
KPHS_p200090	4050..4442	-	393	YP_005229625.1	hypothetical protein	-
KPHS_p200100	4479..5423	-	945	YP_005229626.1	hypothetical protein	-
KPHS_p200110	5449..5568	-	120	YP_005229627.1	hypothetical protein	-
KPHS_p200140	5584..6045	-	462	YP_005229628.1	hypothetical protein	-
KPHS_p200120	6002..6232	+	231	YP_005229629.1	virulence-associated protein	Antitoxin
KPHS_p200130	6229..6645	+	417	YP_005229630.1	putative DNA binding protein	Toxin
KPHS_p200150	6719..8281	+	1563	YP_005229631.1	hypothetical protein	-
KPHS_p200160	8266..9288	+	1023	YP_005229632.1	hypothetical protein	-
KPHS_p200170	9360..9482	+	123	YP_005229633.1	hypothetical protein	-
KPHS_p200180	9592..9819	+	228	YP_005229634.1	putative transposase	-
KPHS_p200190	9864..10241	+	378	YP_005229635.1	putative transposase	-
KPHS_p200200	10243..10623	+	381	YP_005229636.1	IS903D transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaTEM-1B	-	1..111195	111195

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T26032 YP_005229630.1 NC_016846:6229-6645 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T26032 NC_016846:6229-6645 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT26032 YP_005229629.1 NC_016846:6002-6232 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT26032 NC_016846:6002-6232 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3