Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 846018..846672 Replicon chromosome
Accession NZ_CP107001
Organism Escherichia coli strain SY22B5

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag OFB11_RS04150 Protein ID WP_000244777.1
Coordinates 846265..846672 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag OFB11_RS04145 Protein ID WP_000354046.1
Coordinates 846018..846284 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OFB11_RS04120 (841187) 841187..841930 + 744 WP_000951964.1 SDR family oxidoreductase -
OFB11_RS04125 (841987) 841987..843420 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
OFB11_RS04130 (843465) 843465..843776 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
OFB11_RS04135 (843940) 843940..844599 + 660 WP_000250274.1 hemolysin III family protein -
OFB11_RS04140 (844795) 844795..845775 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
OFB11_RS04145 (846018) 846018..846284 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
OFB11_RS04150 (846265) 846265..846672 + 408 WP_000244777.1 protein YgfX Toxin
OFB11_RS04155 (846712) 846712..847233 - 522 WP_001055874.1 flavodoxin FldB -
OFB11_RS04160 (847345) 847345..848241 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
OFB11_RS04165 (848266) 848266..848976 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
OFB11_RS04170 (848982) 848982..850715 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T260280 WP_000244777.1 NZ_CP107001:846265-846672 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T260280 NZ_CP107001:846265-846672 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT260280 WP_000354046.1 NZ_CP107001:846018-846284 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT260280 NZ_CP107001:846018-846284 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References