Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 185670..186205 Replicon plasmid A
Accession NC_016833
Organism Shigella sonnei 53G

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag SSON53G_RS27425 Protein ID WP_000222766.1
Coordinates 185918..186205 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0I0JNN8
Locus tag SSON53G_RS27420 Protein ID WP_001132903.1
Coordinates 185670..185921 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SSON53G_RS27375 180936..181397 + 462 WP_001233866.1 thermonuclease family protein -
SSON53G_RS27380 181443..181652 + 210 WP_001299729.1 hemolysin expression modulator Hha -
SSON53G_RS27385 181691..181936 + 246 Protein_210 hypothetical protein -
SSON53G_RS27395 182705..183058 + 354 Protein_212 DUF2726 domain-containing protein -
SSON53G_RS27400 183298..183558 + 261 WP_000083819.1 replication regulatory protein RepA -
SSON53G_RS27405 183782..183856 + 75 WP_001365705.1 RepA leader peptide Tap -
SSON53G_RS27410 183849..184706 + 858 WP_000130974.1 incFII family plasmid replication initiator RepA -
SSON53G_RS34270 185406..185597 + 192 WP_000829591.1 hypothetical protein -
SSON53G_RS27420 185670..185921 + 252 WP_001132903.1 plasmid stabilization protein Antitoxin
SSON53G_RS27425 185918..186205 + 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
SSON53G_RS27430 186438..187637 - 1200 WP_000947184.1 IS91 family transposase -
SSON53G_RS33245 187647..187835 - 189 WP_000957853.1 hypothetical protein -
SSON53G_RS27440 187957..188115 + 159 Protein_221 transposase -
SSON53G_RS33770 188254..188409 + 156 Protein_222 IS256 family transposase -
SSON53G_RS27450 188439..190166 - 1728 WP_000597727.1 T3SS effector E3 ubiquitin-protein ligase IpaH1.4 -
SSON53G_RS27455 190558..190826 - 269 Protein_224 hypothetical protein -
SSON53G_RS33250 190935..191032 - 98 Protein_225 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospB / ospC3 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ospC1 / ospD3/senA / faeC / faeD / faeE / faeF / faeH / faeI / ipaH7.8 / ipaH4.5 / ospC2 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / tviC / nleE / icsP/sopA 1..215774 215774
- inside IScluster/Tn - ipaH1.4 181951..194441 12490


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T26013 WP_000222766.1 NC_016833:185918-186205 [Shigella sonnei 53G]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T26013 NC_016833:185918-186205 [Shigella sonnei 53G]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCGGCAAAACTAAGAGGGCTAAAGGACTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9225.44 Da        Isoelectric Point: 4.0927

>AT26013 WP_001132903.1 NC_016833:185670-185921 [Shigella sonnei 53G]
MPNIILSETSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT26013 NC_016833:185670-185921 [Shigella sonnei 53G]
ATGCCTAACATCATTCTGTCTGAGACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB A0A0I0JNN8

References