Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5439367..5439992 Replicon chromosome
Accession NZ_CP106964
Organism Klebsiella variicola strain SY1

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag OCU08_RS26250 Protein ID WP_008807903.1
Coordinates 5439367..5439750 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OCU08_RS26255 Protein ID WP_004150355.1
Coordinates 5439750..5439992 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OCU08_RS26235 5436733..5437635 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
OCU08_RS26240 5437632..5438267 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
OCU08_RS26245 5438264..5439193 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
OCU08_RS26250 5439367..5439750 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
OCU08_RS26255 5439750..5439992 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OCU08_RS26260 5440197..5441114 + 918 WP_032741512.1 alpha/beta hydrolase -
OCU08_RS26265 5441129..5442070 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
OCU08_RS26270 5442115..5442552 - 438 WP_032741510.1 D-aminoacyl-tRNA deacylase -
OCU08_RS26275 5442549..5443409 - 861 WP_008807907.1 virulence factor BrkB family protein -
OCU08_RS26280 5443403..5444002 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T260106 WP_008807903.1 NZ_CP106964:c5439750-5439367 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT260106 WP_004150355.1 NZ_CP106964:c5439992-5439750 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References