Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 3416062..3417040 Replicon chromosome
Accession NZ_CP106947
Organism Bacillus thuringiensis strain 4F5

Toxin (Protein)


Gene name spoIISA Uniprot ID W8Y388
Locus tag OAT02_RS17745 Protein ID WP_000624977.1
Coordinates 3416303..3417040 (-) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID W8YZL5
Locus tag OAT02_RS17740 Protein ID WP_000237818.1
Coordinates 3416062..3416190 (-) Length 43 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OAT02_RS17710 (OAT02_17710) 3411580..3412331 + 752 Protein_3437 IS21-like element IS232 family helper ATPase IstB -
OAT02_RS17715 (OAT02_17715) 3412383..3412643 - 261 Protein_3438 hypothetical protein -
OAT02_RS17720 (OAT02_17720) 3412669..3413478 - 810 WP_000678509.1 papain-like cysteine protease family protein -
OAT02_RS17725 (OAT02_17725) 3413721..3415193 - 1473 WP_053513522.1 beta-Ala-His dipeptidase -
OAT02_RS17730 (OAT02_17730) 3415405..3415794 + 390 WP_000713866.1 YxeA family protein -
OAT02_RS17735 (OAT02_17735) 3415813..3415989 - 177 WP_000852629.1 stage II sporulation protein SB -
OAT02_RS17740 (OAT02_17740) 3416062..3416190 - 129 WP_000237818.1 hypothetical protein Antitoxin
OAT02_RS17745 (OAT02_17745) 3416303..3417040 - 738 WP_000624977.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
OAT02_RS17750 (OAT02_17750) 3417278..3418015 - 738 WP_000594152.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
OAT02_RS17755 (OAT02_17755) 3418182..3418664 - 483 WP_000191888.1 MarR family winged helix-turn-helix transcriptional regulator -
OAT02_RS17760 (OAT02_17760) 3418772..3420457 + 1686 WP_074644145.1 alpha-keto acid decarboxylase family protein -
OAT02_RS17765 (OAT02_17765) 3420615..3421397 - 783 WP_000381800.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28307.03 Da        Isoelectric Point: 8.2998

>T260089 WP_000624977.1 NZ_CP106947:c3417040-3416303 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

Antitoxin


Download         Length: 43 a.a.        Molecular weight: 5011.40 Da        Isoelectric Point: 3.9150

>AT260089 WP_000237818.1 NZ_CP106947:c3416190-3416062 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Download         Length: 129 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W8Y388

Antitoxin

Source ID Structure
AlphaFold DB W8YZL5

References