Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 89550..90192 Replicon chromosome
Accession NZ_CP106947
Organism Bacillus thuringiensis strain 4F5

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag OAT02_RS00475 Protein ID WP_000635965.1
Coordinates 89550..89900 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag OAT02_RS00480 Protein ID WP_000004570.1
Coordinates 89905..90192 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OAT02_RS00460 (OAT02_00460) 86486..86944 - 459 WP_000344241.1 SprT family protein -
OAT02_RS00465 (OAT02_00465) 87140..87256 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
OAT02_RS00470 (OAT02_00470) 87314..89482 - 2169 WP_000426236.1 Tex family protein -
OAT02_RS00475 (OAT02_00475) 89550..89900 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
OAT02_RS00480 (OAT02_00480) 89905..90192 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
OAT02_RS00485 (OAT02_00485) 90501..91670 - 1170 WP_053514326.1 alanine racemase -
OAT02_RS00490 (OAT02_00490) 91788..92738 - 951 WP_053514347.1 outer membrane lipoprotein carrier protein LolA -
OAT02_RS00495 (OAT02_00495) 92895..93254 - 360 WP_000583417.1 holo-ACP synthase -
OAT02_RS00500 (OAT02_00500) 93348..93920 + 573 WP_000908523.1 rhomboid family intramembrane serine protease -
OAT02_RS00505 (OAT02_00505) 93913..94875 - 963 WP_000961161.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T260088 WP_000635965.1 NZ_CP106947:c89900-89550 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT260088 WP_000004570.1 NZ_CP106947:c90192-89905 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References