Detailed information of TA system TA260061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4210334..4212017
• Replicon: chromosome
• Accession: NZ_CP106924
• Organism: Escherichia coli strain MFDS1016424

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: OEG87_RS20595
• Protein ID: WP_000361918.1
• Coordinates: 4211100..4212017 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: I2UPX5
• Locus tag: OEG87_RS20590
• Protein ID: WP_000157137.1
• Coordinates: 4210334..4211107 (+)
• Length: 258 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OEG87_RS20575 (4206202)	4206202..4208286	+	2085	WP_001462982.1	protease Lon-related BREX system protein BrxL	-
OEG87_RS20580 (4208405)	4208405..4209754	+	1350	WP_000766442.1	phospholipase D-like domain-containing protein	-
OEG87_RS20585 (4209930)	4209930..4210250	-	321	Protein_4025	IS3 family transposase	-
OEG87_RS20590 (4210334)	4210334..4211107	+	774	WP_000157137.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
OEG87_RS20595 (4211100)	4211100..4212017	+	918	WP_000361918.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
OEG87_RS20600 (4212311)	4212311..4212478	+	168	WP_001356018.1	hypothetical protein	-
OEG87_RS20605 (4212531)	4212531..4213193	-	663	WP_000357928.1	hypothetical protein	-
OEG87_RS20610 (4213266)	4213266..4213907	-	642	Protein_4030	integrase arm-type DNA-binding domain-containing protein	-
OEG87_RS20620 (4214388)	4214388..4215407	+	1020	WP_001389176.1	NADPH-dependent aldehyde reductase Ahr	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34962.10 Da
• Isoelectric Point: 4.9206

>T260061 WP_000361918.1 NZ_CP106924:4211100-4212017 [Escherichia coli]
MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPRLSVDIDLAWIPLESREVALPNVREALTRIAAELKAQ
TGIRAVLQTHNPDEMRIIVTTDDAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYGGKLCAALDRQH
PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMTFEEVSLEELKEVPERMLSTLKT
HFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQWKLRNIGQMSEEKRAQSLEKLEKVMTEWLR

Nucleotide

• Length: 918 bp

Antitoxin

Protein

• Length: 258 a.a.
• Molecular weight: 29333.76 Da
• Isoelectric Point: 9.9889

>AT260061 WP_000157137.1 NZ_CP106924:4210334-4211107 [Escherichia coli]
MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIKLRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLS
SLSWQGRSHYLQLKQNQCWLSVENKAILPKWFKEFPAVEWLMVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRIDTSKINLGSGNRQIVVNGKLNTQ
YQITVPENFTKKGISHG

Nucleotide

• Length: 774 bp

Structures

Antitoxin

• AlphaFold DB: A0A0H0N3W2