Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3591514..3592351 Replicon chromosome
Accession NZ_CP106924
Organism Escherichia coli strain MFDS1016424

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag OEG87_RS17685 Protein ID WP_000227784.1
Coordinates 3591809..3592351 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag OEG87_RS17680 Protein ID WP_001297137.1
Coordinates 3591514..3591825 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OEG87_RS17655 (3586534) 3586534..3587481 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
OEG87_RS17660 (3587503) 3587503..3589494 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
OEG87_RS17665 (3589484) 3589484..3590098 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
OEG87_RS17670 (3590098) 3590098..3590427 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
OEG87_RS17675 (3590439) 3590439..3591329 + 891 WP_000971336.1 heme o synthase -
OEG87_RS17680 (3591514) 3591514..3591825 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
OEG87_RS17685 (3591809) 3591809..3592351 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
OEG87_RS17690 (3592407) 3592407..3593342 - 936 WP_001297127.1 tetratricopeptide repeat protein -
OEG87_RS17695 (3593750) 3593750..3595114 + 1365 WP_001000974.1 MFS transporter -
OEG87_RS17700 (3595242) 3595242..3595733 - 492 WP_001138904.1 nucleotide binding protein YajQ -
OEG87_RS17705 (3595901) 3595901..3596812 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T260058 WP_000227784.1 NZ_CP106924:3591809-3592351 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT260058 WP_001297137.1 NZ_CP106924:3591514-3591825 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References