Detailed information of TA system TA026000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 2512505..2513052
• Replicon: chromosome
• Accession: NC_016830
• Organism: Pseudomonas fluorescens F113

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G8Q8L6
• Locus tag: PSF113_RS41220
• Protein ID: WP_014337667.1
• Coordinates: 2512505..2512780 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G8Q8L7
• Locus tag: PSF113_RS41225
• Protein ID: WP_014337668.1
• Coordinates: 2512780..2513052 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSF113_RS41200	2508842..2509471	+	630	WP_014337664.1	hydrolase	-
PSF113_RS41205	2509722..2510600	+	879	WP_014337665.1	LysR family transcriptional regulator	-
PSF113_RS41210	2510657..2511133	-	477	WP_003203396.1	hemerythrin domain-containing protein	-
PSF113_RS41215	2511332..2512441	-	1110	WP_014337666.1	NAD(P)/FAD-dependent oxidoreductase	-
PSF113_RS41220	2512505..2512780	-	276	WP_014337667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSF113_RS41225	2512780..2513052	-	273	WP_014337668.1	ribbon-helix-helix domain-containing protein	Antitoxin
PSF113_RS41230	2513232..2513567	+	336	WP_014337669.1	GNAT family N-acetyltransferase	-
PSF113_RS41235	2513704..2515167	-	1464	WP_014337670.1	NADH-quinone oxidoreductase subunit NuoN	-
PSF113_RS41240	2515175..2516707	-	1533	WP_014337671.1	NADH-quinone oxidoreductase subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2385173..2582877	197704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10619.17 Da
• Isoelectric Point: 9.5555

>T26000 WP_014337667.1 NC_016830:c2512780-2512505 [Pseudomonas fluorescens F113]
MELKWTSKALSDIARLYEFLAAVNQPAAARTVQQLTAAPTSLLANPRIGERLEEFEPRDVRRIQVGRYEMRYEIAGSTLY
LLRLWHTREDR

Nucleotide

• Length: 276 bp

>T26000 NC_016830:c2512780-2512505 [Pseudomonas fluorescens F113]
ATGGAGTTGAAGTGGACCAGCAAGGCGCTTTCCGACATTGCTCGACTTTATGAATTTCTGGCGGCGGTGAATCAACCCGC
GGCCGCGCGAACAGTGCAACAACTCACGGCCGCGCCGACCTCTCTATTGGCCAACCCGCGCATTGGTGAACGCCTGGAAG
AGTTCGAACCTCGAGACGTGCGCAGAATCCAAGTGGGGCGCTACGAAATGCGCTACGAAATAGCCGGTTCCACCCTGTAC
CTGCTACGCCTGTGGCACACACGTGAGGATCGATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10108.40 Da
• Isoelectric Point: 6.0741

>AT26000 WP_014337668.1 NC_016830:c2513052-2512780 [Pseudomonas fluorescens F113]
MTTTAKTRSVTAHVPVHLAEKVDLMAERLERSRNWIVKQALSAWIEQEEERSRLTHEALADVDAGRVIDHQAVQAWADSL
STDAPLPVPR

Nucleotide

• Length: 273 bp

>AT26000 NC_016830:c2513052-2512780 [Pseudomonas fluorescens F113]
ATGACAACCACCGCGAAAACCCGGTCGGTCACAGCTCACGTCCCGGTGCACCTGGCCGAAAAAGTCGACCTGATGGCCGA
GCGCCTCGAGCGTTCCAGGAACTGGATCGTCAAACAAGCGCTATCTGCCTGGATCGAGCAGGAGGAGGAGCGAAGCCGCC
TGACCCATGAGGCCTTGGCCGATGTTGACGCCGGCCGAGTGATTGACCACCAGGCTGTCCAGGCTTGGGCCGATAGTCTC
AGCACCGACGCCCCGTTACCGGTACCGCGCTGA

Structures

Toxin

• AlphaFold DB: G8Q8L6

Antitoxin

• AlphaFold DB: G8Q8L7