Detailed information of TA system TA025999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2262208..2262752
• Replicon: chromosome
• Accession: NC_016830
• Organism: Pseudomonas fluorescens F113

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: PSF113_RS60920
• Protein ID: WP_044059270.1
• Coordinates: 2262519..2262752 (-)
• Length: 78 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: G8Q5A0
• Locus tag: PSF113_RS40095
• Protein ID: WP_014337468.1
• Coordinates: 2262208..2262516 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSF113_RS40075	2257555..2258748	-	1194	WP_041475826.1	IS4 family transposase	-
PSF113_RS40080	2259147..2260382	+	1236	WP_014337466.1	FYVE zinc finger domain-containing protein	-
PSF113_RS40085	2260516..2261385	-	870	WP_014337467.1	AraC family transcriptional regulator	-
PSF113_RS40090	2261808..2262080	+	273	WP_003203821.1	hypothetical protein	-
PSF113_RS40095	2262208..2262516	-	309	WP_014337468.1	putative addiction module antidote protein	Antitoxin
PSF113_RS60920	2262519..2262752	-	234	WP_044059270.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSF113_RS40105	2263075..2263620	+	546	WP_014337470.1	DUF4410 domain-containing protein	-
PSF113_RS40110	2263795..2264460	-	666	WP_148272449.1	hypothetical protein	-
PSF113_RS40115	2264879..2265079	+	201	WP_013693816.1	hypothetical protein	-
PSF113_RS40120	2265351..2265857	+	507	WP_014337471.1	AAA family ATPase	-
PSF113_RS40125	2266286..2267008	+	723	WP_148272450.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2256164..2279885	23721
	-	flank	IS/Tn	-	-	2257555..2258748	1193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-77)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 78 a.a.
• Molecular weight: 8170.50 Da
• Isoelectric Point: 11.0624

>T25999 WP_044059270.1 NC_016830:c2262752-2262519 [Pseudomonas fluorescens F113]
MRAKNAIARRIDRAAAGNLGDVKPVGDGISELRVDVGAGYRVYFTMRGGVVIVLLAGGDKSSQSADIRRAKKLAKEI

Nucleotide

• Length: 234 bp

>T25999 NC_016830:c2262752-2262519 [Pseudomonas fluorescens F113]
CTGCGAGCAAAAAACGCTATCGCCCGCCGTATTGATCGCGCCGCAGCAGGTAACCTGGGCGATGTGAAACCTGTCGGTGA
TGGTATTTCAGAGTTGCGTGTGGATGTAGGCGCTGGCTACCGGGTCTATTTCACCATGCGCGGTGGAGTGGTCATCGTGC
TACTGGCAGGTGGTGACAAGTCTTCACAGAGTGCTGATATCCGGCGAGCGAAGAAATTGGCAAAGGAGATTTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10988.44 Da
• Isoelectric Point: 5.9574

>AT25999 WP_014337468.1 NC_016830:c2262516-2262208 [Pseudomonas fluorescens F113]
MSQHLTTFDMAALLDSDEAISEYLSQVLADGDSEEFLRAIGYVAKARGMAQIAKDFGMGRESLYKAFAPGSKPRFDTVLK
VIHALGIDLHAQPGHAANHSPA

Nucleotide

• Length: 309 bp

>AT25999 NC_016830:c2262516-2262208 [Pseudomonas fluorescens F113]
ATGAGCCAACACCTGACGACATTTGACATGGCGGCACTGCTCGATAGCGACGAAGCCATCAGTGAATACCTTTCCCAAGT
GCTGGCTGATGGTGATAGCGAGGAGTTTCTCCGAGCGATCGGCTATGTCGCCAAGGCCCGAGGCATGGCGCAGATCGCTA
AAGACTTTGGCATGGGACGAGAAAGCTTGTACAAGGCGTTCGCACCCGGATCAAAGCCGAGATTTGATACCGTACTCAAG
GTCATTCATGCGCTAGGCATTGATCTTCATGCGCAGCCTGGGCATGCCGCTAATCATTCACCTGCCTGA

Structures

Antitoxin

• AlphaFold DB: G8Q5A0