Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4729926..4730423 Replicon chromosome
Accession NC_016822
Organism Shigella sonnei 53G

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag SSON53G_RS24845 Protein ID WP_001173343.1
Coordinates 4730250..4730423 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag SSON53G_RS24840 Protein ID WP_000398619.1
Coordinates 4729926..4730222 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SSON53G_RS24820 (4726657) 4726657..4727376 - 720 WP_000611285.1 two-component system response regulator DcuR -
SSON53G_RS24825 (4727373) 4727373..4729004 - 1632 WP_001216495.1 sensor histidine kinase -
SSON53G_RS24830 (4729185) 4729185..4729415 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
SSON53G_RS24835 (4729427) 4729427..4729699 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
SSON53G_RS24840 (4729926) 4729926..4730222 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
SSON53G_RS24845 (4730250) 4730250..4730423 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
SSON53G_RS24850 (4730542) 4730542..4732059 - 1518 WP_001295085.1 lysine--tRNA ligase -
SSON53G_RS24855 (4732296) 4732296..4733753 - 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
SSON53G_RS24860 (4733812) 4733812..4734069 - 258 Protein_4690 lysine decarboxylase LdcC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T25998 WP_001173343.1 NC_016822:4730250-4730423 [Shigella sonnei 53G]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T25998 NC_016822:4730250-4730423 [Shigella sonnei 53G]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT25998 WP_000398619.1 NC_016822:4729926-4730222 [Shigella sonnei 53G]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT25998 NC_016822:4729926-4730222 [Shigella sonnei 53G]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References