Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5378369..5378994 Replicon chromosome
Accession NZ_CP106890
Organism Klebsiella pneumoniae strain KPN_SINK_001

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag N6138_RS26575 Protein ID WP_002882817.1
Coordinates 5378369..5378752 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag N6138_RS26580 Protein ID WP_004150355.1
Coordinates 5378752..5378994 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N6138_RS26560 (N6138_26555) 5375735..5376637 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
N6138_RS26565 (N6138_26560) 5376634..5377269 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
N6138_RS26570 (N6138_26565) 5377266..5378195 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
N6138_RS26575 (N6138_26570) 5378369..5378752 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
N6138_RS26580 (N6138_26575) 5378752..5378994 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
N6138_RS26585 (N6138_26580) 5379199..5380116 + 918 WP_002882812.1 alpha/beta hydrolase -
N6138_RS26590 (N6138_26585) 5380130..5381071 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
N6138_RS26595 (N6138_26590) 5381116..5381553 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
N6138_RS26600 (N6138_26595) 5381550..5382410 - 861 WP_002882807.1 virulence factor BrkB family protein -
N6138_RS26605 (N6138_26600) 5382404..5383003 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T259965 WP_002882817.1 NZ_CP106890:c5378752-5378369 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT259965 WP_004150355.1 NZ_CP106890:c5378994-5378752 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References