Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2117604..2118151 Replicon chromosome
Accession NZ_CP106838
Organism Klebsiella michiganensis strain K5-3

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0J2H3H5
Locus tag N6B35_RS09965 Protein ID WP_032751830.1
Coordinates 2117604..2117912 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag N6B35_RS09970 Protein ID WP_014227298.1
Coordinates 2117915..2118151 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N6B35_RS09940 (N6B35_09940) 2113520..2113831 - 312 WP_014227304.1 PTS sugar transporter subunit IIB -
N6B35_RS09945 (N6B35_09945) 2114126..2115067 + 942 WP_014227303.1 LacI family DNA-binding transcriptional regulator -
N6B35_RS09950 (N6B35_09950) 2115091..2115384 - 294 WP_047722799.1 YicS family protein -
N6B35_RS09955 (N6B35_09955) 2115594..2116547 + 954 WP_032751828.1 Rpn family recombination-promoting nuclease/putative transposase -
N6B35_RS09960 (N6B35_09960) 2116551..2117453 - 903 WP_009654083.1 DMT family transporter -
N6B35_RS09965 (N6B35_09965) 2117604..2117912 - 309 WP_032751830.1 CcdB family protein Toxin
N6B35_RS09970 (N6B35_09970) 2117915..2118151 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
N6B35_RS09975 (N6B35_09975) 2118257..2119690 - 1434 WP_032751833.1 PTS N-acetylmuramic acid transporter subunit IIBC -
N6B35_RS09980 (N6B35_09980) 2119715..2120617 - 903 WP_032752002.1 N-acetylmuramic acid 6-phosphate etherase -
N6B35_RS09985 (N6B35_09985) 2120778..2121944 - 1167 WP_014227296.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11682.54 Da        Isoelectric Point: 6.7155

>T259880 WP_032751830.1 NZ_CP106838:c2117912-2117604 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQLEVVKASMDFLFDGI

Download         Length: 309 bp

>T259880 NZ_CP106838:c2117912-2117604 [Klebsiella michiganensis]
ATGCAGTATTACGTATATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTGAAGAG
GTTTGTAATCTGAATCACCAGCTCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT259880 WP_014227298.1 NZ_CP106838:c2118151-2117915 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT259880 NZ_CP106838:c2118151-2117915 [Klebsiella michiganensis]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2H3H5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References