Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 1289307..1289883 Replicon chromosome
Accession NZ_CP106838
Organism Klebsiella michiganensis strain K5-3

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag N6B35_RS06065 Protein ID WP_080528481.1
Coordinates 1289596..1289883 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0H3H591
Locus tag N6B35_RS06060 Protein ID WP_014227757.1
Coordinates 1289307..1289609 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N6B35_RS06045 (N6B35_06045) 1286146..1286481 + 336 WP_080528483.1 endoribonuclease SymE -
N6B35_RS06050 (N6B35_06050) 1286935..1287846 + 912 WP_080528482.1 acetamidase/formamidase family protein -
N6B35_RS06055 (N6B35_06055) 1287843..1289186 + 1344 WP_049101852.1 APC family permease -
N6B35_RS06060 (N6B35_06060) 1289307..1289609 - 303 WP_014227757.1 BrnA antitoxin family protein Antitoxin
N6B35_RS06065 (N6B35_06065) 1289596..1289883 - 288 WP_080528481.1 BrnT family toxin Toxin
N6B35_RS06070 (N6B35_06070) 1290143..1290586 - 444 WP_014227755.1 FosA family fosfomycin resistance glutathione transferase -
N6B35_RS06075 (N6B35_06075) 1290580..1291488 - 909 WP_049080873.1 LysR family transcriptional regulator -
N6B35_RS06080 (N6B35_06080) 1291576..1292358 + 783 WP_025108473.1 NAD(P)H-dependent oxidoreductase -
N6B35_RS06085 (N6B35_06085) 1292506..1293090 + 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -
N6B35_RS06090 (N6B35_06090) 1293108..1293908 + 801 WP_014227752.1 winged helix-turn-helix domain-containing protein -
N6B35_RS06095 (N6B35_06095) 1293905..1294423 + 519 WP_064380989.1 FidL-like protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1278169..1289886 11717


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(38-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11104.61 Da        Isoelectric Point: 8.5901

>T259879 WP_080528481.1 NZ_CP106838:c1289883-1289596 [Klebsiella michiganensis]
MPMEFEWDANKAISNLRKHGIRFEEAVLVLDDPRHLSRQDRYENGAYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11607.18 Da        Isoelectric Point: 9.4429

>AT259879 WP_014227757.1 NZ_CP106838:c1289609-1289307 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H591

References