Detailed information of TA system TA025982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 443590..444427
• Replicon: chromosome
• Accession: NC_016822
• Organism: Shigella sonnei 53G

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: SSON53G_RS02185
• Protein ID: WP_000227791.1
• Coordinates: 443590..444132 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: SSON53G_RS02190
• Protein ID: WP_001297137.1
• Coordinates: 444116..444427 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SSON53G_RS02155 (438654)	438654..439145	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
SSON53G_RS02160 (439273)	439273..440637	-	1365	WP_001000971.1	MFS transporter	-
SSON53G_RS02165 (440756)	440756..441453	+	698	WP_237158176.1	IS1-like element IS1A family transposase	-
SSON53G_RS02170 (441822)	441822..442220	+	399	Protein_408	SEL1-like repeat protein	-
SSON53G_RS02180 (442968)	442968..443534	+	567	Protein_410	SEL1-like repeat protein	-
SSON53G_RS02185 (443590)	443590..444132	-	543	WP_000227791.1	GNAT family N-acetyltransferase	Toxin
SSON53G_RS02190 (444116)	444116..444427	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
SSON53G_RS02195 (444612)	444612..445502	-	891	WP_000971327.1	heme o synthase	-
SSON53G_RS02200 (445514)	445514..445843	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
SSON53G_RS02205 (445843)	445843..446457	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
SSON53G_RS02210 (446447)	446447..448438	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
SSON53G_RS02215 (448460)	448460..449407	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19794.98 Da
• Isoelectric Point: 8.3395

>T25982 WP_000227791.1 NC_016822:c444132-443590 [Shigella sonnei 53G]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNSVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T25982 NC_016822:c444132-443590 [Shigella sonnei 53G]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACTCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT25982 WP_001297137.1 NC_016822:c444427-444116 [Shigella sonnei 53G]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT25982 NC_016822:c444427-444116 [Shigella sonnei 53G]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3