Detailed information of TA system TA025980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 152947..153854
• Replicon: plasmid pPANA10
• Accession: NC_016817
• Organism: Pantoea ananatis LMG 5342

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: PANA5342_RS44590
• Protein ID: WP_014333139.1
• Coordinates: 152947..153414 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A8A4JZ23
• Locus tag: PANA5342_RS44595
• Protein ID: WP_014598335.1
• Coordinates: 153411..153854 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PANA5342_RS45995	148334..148567	-	234	WP_014333135.1	DUF333 domain-containing protein	-
PANA5342_RS44575	148655..149227	+	573	WP_014598338.1	NAD(P)H-dependent oxidoreductase	-
PANA5342_RS44580	149289..150224	-	936	WP_013027859.1	hypothetical protein	-
PANA5342_RS44585	150908..152302	-	1395	WP_014333136.1	flagellar filament capping protein FliD	-
PANA5342_RS44590	152947..153414	-	468	WP_014333139.1	RES family NAD+ phosphorylase	Toxin
PANA5342_RS44595	153411..153854	-	444	WP_014598335.1	DUF2384 domain-containing protein	Antitoxin
PANA5342_RS46000	154241..154465	+	225	WP_013027863.1	KTSC domain-containing protein	-
PANA5342_RS44600	154619..155314	+	696	WP_014333143.1	hypothetical protein	-
PANA5342_RS44605	155389..156078	-	690	WP_014598333.1	HTH-type transcriptional regulator RutR	-
PANA5342_RS44610	156477..157569	+	1093	Protein_133	pyrimidine utilization protein A	-
PANA5342_RS44615	157566..158318	+	753	WP_019105823.1	pyrimidine utilization protein B	-
PANA5342_RS44620	158344..158730	+	387	WP_013027868.1	pyrimidine utilization protein C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fliC / fliC	1..302599	302599

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (2-145)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (31-89)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17211.69 Da
• Isoelectric Point: 5.1570

>T25980 WP_014333139.1 NC_016817:c153414-152947 [Pantoea ananatis LMG 5342]
MKLYRMTKTRYLGSAWSGFGAREGGGRWNSVGVSMVYASETASLTMLETLIHLQSASVLDFFTLMSIDVPDRLIEWIDIK
QLPDDWAATEAPAALRLFGDAWIQSGGSVALRVPSALSPVESNYLLNPEHPEFSAIVRQAVNIPFLFDARFSRQV

Nucleotide

• Length: 468 bp

>T25980 NC_016817:c153414-152947 [Pantoea ananatis LMG 5342]
TTGAAGCTCTATCGCATGACCAAAACGCGCTATCTAGGGTCGGCCTGGAGCGGTTTTGGTGCCCGTGAAGGCGGCGGACG
CTGGAACAGCGTCGGCGTATCGATGGTCTACGCTTCTGAAACTGCTTCCCTCACCATGCTGGAGACGCTTATCCATCTGC
AAAGCGCCAGCGTACTGGACTTTTTTACCCTCATGAGCATTGACGTTCCCGACAGGCTGATTGAATGGATTGATATCAAG
CAACTGCCTGACGACTGGGCCGCAACGGAAGCGCCTGCAGCGCTGCGTTTATTTGGTGATGCCTGGATACAAAGCGGAGG
ATCGGTCGCACTTCGCGTGCCCAGTGCCCTTTCGCCCGTGGAATCGAATTATCTTCTCAATCCCGAGCATCCTGAATTCA
GTGCGATCGTCAGACAGGCGGTAAACATCCCCTTCCTTTTTGACGCACGTTTTTCCCGACAGGTATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16375.69 Da
• Isoelectric Point: 7.0208

>AT25980 WP_014598335.1 NC_016817:c153854-153411 [Pantoea ananatis LMG 5342]
MKQFTLPVSAAEPDRLWQFAGLSSADGVMLMDQIDDGLDGTVASRIVTWAQISQAELRRMTGIAGSTFTRGVKSRFTAEQ
SERLVRFIRVMDRAVELFEGDKEKARQWLNEPARALGNKKPADLVASETGAYEVLKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT25980 NC_016817:c153854-153411 [Pantoea ananatis LMG 5342]
ATGAAACAGTTCACTTTACCCGTATCCGCTGCAGAACCGGATCGTCTATGGCAATTTGCTGGCCTGAGCAGTGCTGACGG
CGTGATGCTGATGGATCAAATCGATGATGGTCTGGACGGTACGGTAGCCAGTCGCATTGTCACCTGGGCGCAAATCTCTC
AGGCAGAACTGCGCCGTATGACCGGCATTGCGGGCTCAACCTTCACCCGCGGCGTTAAAAGCCGCTTTACTGCGGAGCAG
AGCGAACGGCTGGTGCGTTTTATTCGCGTGATGGATCGTGCGGTTGAGCTGTTTGAGGGCGATAAAGAAAAGGCACGGCA
GTGGCTCAATGAGCCTGCGCGCGCCTTAGGCAATAAAAAACCGGCCGATCTGGTTGCGTCAGAAACCGGAGCTTATGAAG
TGCTGAAACTCATTACCCGGCTTGAACACGGTGTGTATTCTTGA