Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1371578..1372415 Replicon chromosome
Accession NZ_CP106756
Organism Escherichia coli strain B-JRPT-4119

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag N8153_RS06585 Protein ID WP_000227786.1
Coordinates 1371578..1372120 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag N8153_RS06590 Protein ID WP_001297137.1
Coordinates 1372104..1372415 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N8153_RS06565 (1367108) 1367108..1368019 - 912 WP_000705853.1 2-dehydropantoate 2-reductase -
N8153_RS06570 (1368187) 1368187..1368678 + 492 WP_001138904.1 nucleotide binding protein YajQ -
N8153_RS06575 (1368806) 1368806..1370170 - 1365 WP_001000978.1 MFS transporter -
N8153_RS06580 (1370587) 1370587..1371522 + 936 WP_001297127.1 tetratricopeptide repeat protein -
N8153_RS06585 (1371578) 1371578..1372120 - 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
N8153_RS06590 (1372104) 1372104..1372415 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
N8153_RS06595 (1372600) 1372600..1373490 - 891 WP_000971336.1 heme o synthase -
N8153_RS06600 (1373502) 1373502..1373831 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
N8153_RS06605 (1373831) 1373831..1374445 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
N8153_RS06610 (1374435) 1374435..1376426 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
N8153_RS06615 (1376448) 1376448..1377395 - 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T259713 WP_000227786.1 NZ_CP106756:c1372120-1371578 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT259713 WP_001297137.1 NZ_CP106756:c1372415-1372104 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References