Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 883342..883918 Replicon chromosome
Accession NZ_CP106756
Organism Escherichia coli strain B-JRPT-4119

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag N8153_RS04210 Protein ID WP_001297643.1
Coordinates 883342..883629 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag N8153_RS04215 Protein ID WP_000063156.1
Coordinates 883616..883918 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N8153_RS04190 (879595) 879595..880875 - 1281 WP_001037380.1 DUF445 domain-containing protein -
N8153_RS04195 (880916) 880916..882148 - 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
N8153_RS04200 (882294) 882294..882389 - 96 Protein_806 dienelactone hydrolase family protein -
N8153_RS04205 (882446) 882446..883143 + 698 Protein_807 IS1 family transposase -
N8153_RS04210 (883342) 883342..883629 + 288 WP_001297643.1 BrnT family toxin Toxin
N8153_RS04215 (883616) 883616..883918 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
N8153_RS04220 (883952) 883952..884908 - 957 WP_001297640.1 GTPase -
N8153_RS04225 (884919) 884919..885122 - 204 WP_000467859.1 YbdD/YjiX family protein -
N8153_RS04230 (885172) 885172..887322 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T259710 WP_001297643.1 NZ_CP106756:883342-883629 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT259710 WP_000063156.1 NZ_CP106756:883616-883918 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References