Detailed information of TA system TA025967

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 1148605..1149338
• Replicon: chromosome
• Accession: NC_016816
• Organism: Pantoea ananatis LMG 5342

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: PANA5342_RS28080
• Protein ID: WP_015699698.1
• Coordinates: 1148605..1148934 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: PANA5342_RS28085
• Protein ID: WP_015699699.1
• Coordinates: 1148973..1149338 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PANA5342_RS28065	1143841..1144305	-	465	WP_015699695.1	hypothetical protein	-
PANA5342_RS28070	1144913..1145917	-	1005	WP_015699696.1	hypothetical protein	-
PANA5342_RS28075	1146317..1147729	-	1413	WP_041455837.1	hypothetical protein	-
PANA5342_RS46185	1148043..1148222	-	180	Protein_1053	integrase	-
PANA5342_RS28080	1148605..1148934	-	330	WP_015699698.1	TA system toxin CbtA family protein	Toxin
PANA5342_RS28085	1148973..1149338	-	366	WP_015699699.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
PANA5342_RS28090	1149364..1149585	-	222	WP_015699700.1	DUF987 family protein	-
PANA5342_RS28095	1149582..1150124	-	543	WP_015699701.1	DNA repair protein RadC	-
PANA5342_RS28100	1150137..1150580	-	444	WP_015699702.1	antirestriction protein	-
PANA5342_RS28105	1150611..1151432	-	822	WP_015699703.1	DUF945 domain-containing protein	-
PANA5342_RS28110	1151531..1151761	-	231	WP_041455838.1	DUF905 domain-containing protein	-
PANA5342_RS28115	1151833..1152282	-	450	WP_015699704.1	hypothetical protein	-
PANA5342_RS28120	1152279..1152731	-	453	WP_015699705.1	hypothetical protein	-
PANA5342_RS28125	1152768..1153337	-	570	WP_015699706.1	hypothetical protein	-
PANA5342_RS28130	1153337..1154041	-	705	WP_015699707.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1145993..1169883	23890

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12227.97 Da
• Isoelectric Point: 6.4757

>T25967 WP_015699698.1 NC_016816:c1148934-1148605 [Pantoea ananatis LMG 5342]
MQTISSHPTRAAQPCLSPVEIWQLLLIHLLSQHYGLTLNDTPFSDEATIQEHIDAGISLSDAVNFLVEKYGLVRIDRKGF
SWQEQTPSLSVVDILRARRSTGLLKTDVK

Nucleotide

• Length: 330 bp

>T25967 NC_016816:c1148934-1148605 [Pantoea ananatis LMG 5342]
ATGCAAACCATATCATCACACCCGACACGGGCGGCTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGTTGCTGCTGAT
TCATCTGTTATCGCAGCACTATGGCCTGACGCTGAATGATACACCGTTCAGTGATGAAGCTACCATCCAGGAACATATTG
ATGCAGGAATATCGCTCAGCGATGCGGTGAACTTTCTGGTAGAAAAATACGGGCTTGTCCGTATCGACCGGAAAGGATTT
TCGTGGCAAGAGCAGACTCCGTCTCTTTCCGTAGTGGATATTCTGCGAGCAAGGCGCTCTACAGGCTTGCTAAAAACTGA
CGTGAAATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13490.21 Da
• Isoelectric Point: 5.4580

>AT25967 WP_015699699.1 NC_016816:c1149338-1148973 [Pantoea ananatis LMG 5342]
MNDHSESGTKPENPAIQQWGLKRDLTPCFGARLVQEGNHLHYLADRAGFSGAFTDDVAMRLDQAFPLMMKQLELMLTSGE
LNPRHPHSVTLYHNGLTCEANTLGSCGYVFIAIYPEQTEPQ

Nucleotide

• Length: 366 bp

>AT25967 NC_016816:c1149338-1148973 [Pantoea ananatis LMG 5342]
ATGAACGATCACTCAGAATCCGGTACCAAGCCGGAGAATCCCGCCATCCAGCAGTGGGGACTGAAGCGTGATTTAACACC
GTGTTTTGGCGCACGTCTGGTTCAGGAGGGCAACCATCTGCATTATCTGGCTGACCGAGCAGGGTTTAGCGGGGCGTTCA
CTGACGATGTTGCAATGCGCCTCGACCAGGCATTTCCGCTCATGATGAAGCAACTTGAGCTGATGCTGACCAGTGGAGAG
CTCAACCCTAGGCACCCGCACAGCGTCACGCTTTACCACAACGGACTCACCTGCGAAGCCAACACACTCGGCTCCTGTGG
CTATGTCTTCATTGCTATCTATCCTGAGCAGACTGAGCCGCAGTAA