Detailed information of TA system TA025965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/-
• Location: 4806232..4806808
• Accession: NC_016810
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: SL1344_RS23235
• Protein ID: WP_001131963.1
• Coordinates: 4806232..4806519 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: SL1344_RS23240
• Protein ID: WP_000063142.1
• Coordinates: 4806506..4806808 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SL1344_RS23225 (SL1344_4457)	4801342..4804851	-	3510	WP_001043484.1	type I restriction-modification system endonuclease	-
SL1344_RS23230 (SL1344_4458)	4805049..4805963	+	915	WP_000290512.1	restriction endonuclease	-
SL1344_RS23235 (SL1344_4459)	4806232..4806519	+	288	WP_001131963.1	BrnT family toxin	Toxin
SL1344_RS23240 (SL1344_4460)	4806506..4806808	+	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
SL1344_RS23245 (SL1344_4461)	4806883..4807839	-	957	WP_000187839.1	GTPase	-
SL1344_RS23250 (SL1344_4462)	4807850..4808053	-	204	WP_000460616.1	YbdD/YjiX family protein	-
SL1344_RS23255 (SL1344_4463)	4808148..4810298	-	2151	WP_000379927.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T25965 WP_001131963.1 NC_016810:4806232-4806519 [Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T25965 NC_016810:4806232-4806519 [Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT25965 WP_000063142.1 NC_016810:4806506-4806808 [Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT25965 NC_016810:4806506-4806808 [Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01