259623

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1331858..1332774 Replicon chromosome
Accession NZ_CP106673
Organism Bacillus subtilis strain SRCM116268

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag N7985_RS06995 Protein ID WP_003244695.1
Coordinates 1332028..1332774 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag N7985_RS06990 Protein ID WP_003232646.1
Coordinates 1331858..1332028 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7985_RS06955 (1328721) 1328721..1329050 + 330 WP_041850928.1 XkdW family protein -
N7985_RS06960 (1329047) 1329047..1329211 + 165 WP_041850927.1 XkdX family protein -
N7985_RS06965 (1329255) 1329255..1330094 + 840 WP_041850926.1 phage-like element PBSX protein XepA -
N7985_RS06970 (1330147) 1330147..1330416 + 270 WP_003232655.1 hemolysin XhlA family protein -
N7985_RS06975 (1330429) 1330429..1330692 + 264 WP_003232653.1 phage holin -
N7985_RS06980 (1330705) 1330705..1331598 + 894 WP_041850925.1 N-acetylmuramoyl-L-alanine amidase -
N7985_RS06985 (1331635) 1331635..1331772 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
N7985_RS06990 (1331858) 1331858..1332028 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
N7985_RS06995 (1332028) 1332028..1332774 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
N7985_RS07000 (1332884) 1332884..1333885 - 1002 WP_003232642.1 inorganic phosphate transporter -
N7985_RS07005 (1333898) 1333898..1334515 - 618 WP_003218470.1 DUF47 domain-containing protein -
N7985_RS07010 (1334791) 1334791..1336107 - 1317 WP_041850924.1 serine/threonine exchanger -
N7985_RS07015 (1336495) 1336495..1337445 + 951 WP_041344800.1 ring-cleaving dioxygenase -
N7985_RS07020 (1337546) 1337546..1337692 + 147 WP_120363335.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1296386..1353274 56888


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T259623 WP_003244695.1 NZ_CP106673:c1332774-1332028 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT259623 WP_003232646.1 NZ_CP106673:c1332028-1331858 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References