Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3583263..3583899 Replicon chromosome
Accession NZ_CP106671
Organism Bacillus subtilis strain SRCM116301

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag N7980_RS18515 Protein ID WP_003156187.1
Coordinates 3583263..3583613 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag N7980_RS18520 Protein ID WP_003225183.1
Coordinates 3583618..3583899 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7980_RS18475 (3578307) 3578307..3578906 - 600 WP_003246608.1 phosphoserine phosphatase RsbX -
N7980_RS18480 (3578906) 3578906..3579694 - 789 WP_003246715.1 RNA polymerase sigma factor SigB -
N7980_RS18485 (3579660) 3579660..3580142 - 483 WP_003234299.1 anti-sigma B factor RsbW -
N7980_RS18490 (3580139) 3580139..3580468 - 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
N7980_RS18495 (3580530) 3580530..3581537 - 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
N7980_RS18500 (3581549) 3581549..3581950 - 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
N7980_RS18505 (3581954) 3581954..3582319 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
N7980_RS18510 (3582324) 3582324..3583148 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
N7980_RS18515 (3583263) 3583263..3583613 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
N7980_RS18520 (3583618) 3583618..3583899 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
N7980_RS18525 (3584015) 3584015..3585184 - 1170 WP_015252766.1 alanine racemase -
N7980_RS18530 (3585298) 3585298..3586314 - 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
N7980_RS18535 (3586480) 3586480..3586845 - 366 WP_015252768.1 holo-ACP synthase -
N7980_RS18540 (3586940) 3586940..3587539 + 600 WP_041850724.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T259621 WP_003156187.1 NZ_CP106671:c3583613-3583263 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT259621 WP_003225183.1 NZ_CP106671:c3583899-3583618 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References